DIFFERENTIAL EXPRESSION OF SNAP-25 PROTEIN ISOFORMS DURING DIVERGENT VESICLE FUSION EVENTS OF NEURAL DEVELOPMENT
DIFFERENTIAL EXPRESSION OF SNAP-25 PROTEIN ISOFORMS DURING DIVERGENT VESICLE FUSION EVENTS OF NEURAL DEVELOPMENT
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DOI：
10.1073/pnas.92.5.1510
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发表时间：
1995-02-28
影响因子：
11.1
通讯作者：
WILSON, MC
中科院分区：
文献类型：
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作者：
BARK, IC;HAHN, KM;WILSON, MC
突触前质膜蛋白SNAP-25（25 kDa的突触体相关蛋白）已被牵连的几个神经特异性组件，直接组成性融合机制的调节囊泡运输和胞吐神经递质释放之一。存在SNAP-25的两种选择性剪接同种型a和B，它们在推定的膜相互作用结构域上不同。我们发现，这两种亚型有不同的定量和解剖模式的表达在大脑发育过程中，在神经元，神经内分泌细胞和蛋白质定位不同的转染PC 12嗜铬细胞瘤细胞的神经突。这些研究结果表明，SNAP-25的替代亚型可能在轴突生长过程中增加膜和释放神经调节肽和神经递质所需的囊泡融合事件中发挥不同的作用。
The presynaptic plasma membrane protein SNAP-25 (synaptosome-associated protein of 25 kDa) has been implicated as one of several neural-specific components that direct constitutive fusion mechanisms to the regulated vesicle trafficking and exocytosis of neurotransmitter release. There exist two alternatively spliced isoforms of SNAP-25, a and b, which differ in a putative membrane-interacting domain. We show that these two isoforms have distinct quantitative and anatomical patterns of expression during brain development, in neurons, and in neuroendocrine cells and that the proteins localize differently in neurites of transfected PC12 pheochromocytoma cells. These findings indicate that alternative isoforms of SNAP-25 may play distinct roles in vesicular fusion events required for membrane addition during axonal outgrowth and for release of neuromodulatory peptides and neurotransmitters.