S100 protein-like immunoreactivity in the crypt olfactory neurons of the adult zebrafish
S100 protein-like immunoreactivity in the crypt olfactory neurons of the adult zebrafish
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DOI：
10.1016/j.neulet.2004.08.077
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发表时间：
2004-11-23
影响因子：
2.5
通讯作者：
Vega, JA
中科院分区：
文献类型：
--
作者：
Germanà, A;Montalbano, G;Vega, JA
某些硬骨鱼（包括斑马鱼）的嗅觉上皮包含三种类型的嗅觉感觉神经元。由于斑马鱼已成为研究嗅觉系统神经发生的理想模型，因此识别不同神经元群体的特异性标记具有重要意义。本研究采用免疫组织化学方法分析了S100样蛋白在成年斑马鱼嗅上皮中的分布。令人惊讶的是，特异性S100蛋白样免疫染色仅在隐窝神经元中检测到，而纤毛和微绒毛神经元以及支持的胶质细胞没有反应。免疫染色模式仅为胞浆，胞体内无明显极性，免疫染色强度与神经元成熟期无关。S100蛋白在隐窝嗅觉神经元中的作用尚不清楚，尽管它可能与这些细胞对化学刺激的反应能力有关。在任何情况下，它都是识别斑马鱼隐窝嗅觉神经元的一个很好的标记。2004爱思唯尔爱尔兰有限公司版权所有。
The olfactory epithelium of some teleosts, including zebrafish, contains three types of olfactory sensory neurons. Because zebrafish has become an ideal model for the study of neurogenesis in the olfactory system, it is of capital importance the identification of specific markers for different neuronal populations. In this study we used immunohistochemistry to analyze the distribution of S100 protein-like in the adult zebrafish olfactory epithelium. Surprisingly, specific S100 protein-like immunostaining was detected exclusively in crypt neurons, whereas ciliated and microvillous neurons were not reactive, and the supporting glial cells as well. The pattern of immunostaining was exclusively cytoplasmic without apparent polarity within the soma, and the intensity of immunostaining was not related with the maturative stage of the neurons. The role of S100 protein in crypt olfactory neurons is unknown, although it is probably associated with the capacity of these cells to respond to chemical stimuli. In any case, it represents an excellent marker to identify crypt olfactory neurons in zebrafish. (C) 2004 Elsevier Ireland Ltd. All rights reserved.