Treatment of Oral Multispecies Biofilms by an Anti-Biofilm Peptide.
Treatment of Oral Multispecies Biofilms by an Anti-Biofilm Peptide.
复制标题
DOI：
10.1371/journal.pone.0132512
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hancock RE
中科院分区：
文献类型：
--
作者：
Wang Z;de la Fuente-Núñez C;Shen Y;Haapasalo M;Hancock RE
口腔生物膜是多种微生物群落，对抗菌剂表现出较高的耐药性。牙菌斑引起高度流行和代价高昂的与生物膜相关的口腔感染，从而导致龋齿或其他类型的口腔感染。我们研究了最近发现的抗生物被膜肽1018诱导杀死口腔多物种生物膜内细菌细胞的能力。在10μg/ml(6.5μM)浓度下，多肽10 18对生物被膜的形成有明显的抑制作用(p<0.0 5)。共聚焦显微镜显示，10μg/ml1018在3天内杀死60%以上的菌斑生物膜细胞群，而在5μg/ml时，50%的细胞死亡，在1μg/ml时，引发约30%的细菌总数的细胞死亡，因此该多肽在预制生物膜上的活性具有浓度依赖性。唾液的存在不影响多肽的活性，因为多肽1018在唾液涂层和非唾液涂层的羟基磷灰石表面杀灭口腔生物膜的能力没有统计学上的显著差异。扫描电子显微镜实验表明，1018肽可诱导菌斑生物膜中的细胞溶解。此外，与单独使用时相比，多肽1018和洗必泰(CHX)的联合使用增加了每种化合物的抗生物被膜活性，导致50%的生物膜被杀死，35%的生物膜在3分钟内被分散。多肽1018可单独使用或与CHX联合使用，作为一种无毒、有效的抗生物被膜剂用于临床牙科菌斑消毒。
Human oral biofilms are multispecies microbial communities that exhibit high resistance to antimicrobial agents. Dental plaque gives rise to highly prevalent and costly biofilm-related oral infections, which lead to caries or other types of oral infections. We investigated the ability of the recently identified anti-biofilm peptide 1018 to induce killing of bacterial cells present within oral multispecies biofilms. At 10 μg/ml (6.5 μM), peptide 1018 was able to significantly (p<0.05) prevent biofilm formation over 3 days. The activity of the peptide on preformed biofilms was found to be concentration-dependent since more than 60% of the total plaque biofilm cell population was killed by 10 μg/ml of peptide 1018 in 3 days, while at 5 μg/ml 50% of cells were dead and at 1 μg/ml the peptide triggered cell death in around 30% of the total bacterial population, as revealed by confocal microscopy. The presence of saliva did not affect peptide activity, since no statistically significant difference was found in the ability of peptide 1018 to kill oral biofilms using either saliva coated and non-saliva coated hydroxyapatite surfaces. Scanning electron microscopy experiments indicated that peptide 1018 induced cell lysis in plaque biofilms. Furthermore, combined treatment using peptide 1018 and chlorhexidine (CHX) increased the anti-biofilm activity of each compound compared to when these were used alone, resulting in >50% of the biofilm being killed and >35% being dispersed in only 3 minutes. Peptide 1018 may potentially be used by itself or in combination with CHX as a non-toxic and effective anti-biofilm agent for plaque disinfection in clinical dentistry.