Relationship of Circulating CXCR4(+) EPC with Prognosis of Mild Traumatic Brain Injury Patients.
Relationship of Circulating CXCR4(+) EPC with Prognosis of Mild Traumatic Brain Injury Patients.
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DOI：
10.14336/ad.2016.0610
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发表时间：
2017-02
影响因子：
7.4
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Lin Y;Luo LL;Sun J;Gao W;Tian Y;Park E;Baker A;Chen J;Jiang R;Zhang J
探讨轻度颅脑损伤（TBI）患者循环内皮祖细胞（EPC）和基质细胞衍生因子-1α（SDF-1α）/CXCR4表达的变化以及EPC水平与轻度TBI预后的相关性。纳入 72 名 TBI 患者（57 名轻度 TBI 患者，15 名中度 TBI 患者）和 25 名健康受试者（对照）。采用流式细胞仪计数TBI后1、4、7、14、21天循环EPCs、CD34+和CD133+细胞的数量以及各细胞群中CXCR4+细胞的百分比。采用ELISA法检测血清中SDF-1α水平。 TBI后3个月根据扩展格拉斯哥结局量表和日常生活能力量表将患者分为预后不良组和良好预后组。对各检测指标与轻度TBI预后进行相关性分析。中度TBI患者SDF-1α、CXCR4表达水平高于轻度TBI患者（P < 0.05）。 TBI后第7天CXCR4+ EPC的比例在预后不良的轻度TBI患者中明显高于预后良好的患者（P < 0.05）。轻度TBI患者早期HAMA和HAMD评分显着低于中度TBI患者（P < 0.05）。 TBI 后第 7 天的 CXCR4+ EPC 百分比与 3 个月的预后结果显着相关。轻度 TBI 可以诱导循环 EPC 的动员。 TBI后7天EPCs中CXCR4+的表达反映了脑损伤的短期预后，可能成为预测轻度TBI预后的潜在生物学标志物。
To investigate the changes of circulating endothelial progenitor cells (EPCs) and stromal cell-derived factor-1α (SDF-1α)/CXCR4 expression in patients with mild traumatic brain injury (TBI) and the correlation between EPC level and the prognosis of mild TBI. 72 TBI patients (57 mild TBI, 15 moderate TBI patients) and 25 healthy subjects (control) were included. The number of circulating EPCs, CD34+, and CD133+ cells and the percentage of CXCR4+ cells in each cell population at 1,4,7,14,21 days after TBI were counted by flow cytometer. SDF-1α levels in serum were detected by ELISA assay. The patients were divided into poor and good prognosis groups based on Extended Glasgow Outcome Scale and Activity of Daily Living Scale at 3 months after TBI. Correlation analysis between each detected index and prognosis of mild TBI was performed. Moderate TBI patients have higher levels of SDF-1α and CXCR4 expression than mild TBI patients (P < 0.05). The percentage of CXCR4+ EPCs at day 7 post-TBI was significantly higher in mild TBI patients with poor prognosis than the ones with good prognosis (P < 0.05). HAMA and HAMD scores in mild TBI patients were significantly lower than moderate TBI patients (P < 0.05) in early term. The percentage of CXCR4+ EPCs at day 7 after TBI was significantly correlated with the prognosis outcome at 3 months. The mobilization of circulating EPCs can be induced in mild TBI. The expression of CXCR4+ in EPCs at 7 days after TBI reflects the short-term prognosis of brain injury, and could be a potential biological marker for prognosis prediction of mild TBI.