Recovering circulating extracellular or cell-free RNA from bodily fluids
Recovering circulating extracellular or cell-free RNA from bodily fluids
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DOI：
10.1016/j.canep.2011.02.016
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发表时间：
2011-12-01
影响因子：
2.6
通讯作者：
Kouidou, Sofia
中科院分区：
文献类型：
--
作者：
Tzimagiorgis, Georgios;Michailidou, Evangelia Z.;Kouidou, Sofia
生物体液中细胞外循环或无细胞RNA的存在正成为一种有前途的无创和经济有效的癌症检测诊断工具。细胞外RNA或miRNA作为生物标志物既可用于疾病的早期检测和诊断，也可作为复发模式和监测的标志物。在这篇综述文章中，我们参考了循环细胞外RNA的来源，我们总结了生物体液(血清/血浆、唾液、尿液、脑脊液和支气管灌洗液)中含有大量循环细胞外（或无细胞）RNA的各种类型恶性肿瘤患者，我们讨论了为获得用于RNA表达分析的大多数实验方法的高质量和完整性的RNA材料所需使用的适当试剂和方法。此外，我们讨论了RT-PCR或微阵列方法的优点和缺点，这些方法更常用于细胞外RNA分析过程。(C) 2011 Elsevier Ltd.版权所有。
The presence of extracellular circulating or cell-free RNA in biological fluids is becoming a promising diagnostic tool for non invasive and cost effective cancer detection. Extracellular RNA or miRNA as biological marker could be used either for the early detection and diagnosis of the disease or as a marker of recurrence patterns and surveillance. In this review article, we refer to the origin of the circulating extracellular RNA, we summarise the data on the biological fluids (serum/plasma, saliva, urine, cerebrospinal fluid and bronchial lavage fluid) of patients suffering from various types of malignancies reported to contain a substantial amount of circulating extracellular (or cell-free) RNAs and we discuss the appropriate reagents and methodologies needed to be employed in order to obtain RNA material of high quality and integrity for the majority of the experimental methods used in RNA expression analysis. Furthermore, we discuss the advantages and disadvantages of the RT-PCR or microarray methodology which are the methods more often employed in procedures of extracellular RNA analysis. (C) 2011 Elsevier Ltd. All rights reserved.