Inhibition of Herpes Simplex Virus Type 1 Infection by Silver Nanoparticles Capped with Mercaptoethane Sulfonate
Inhibition of Herpes Simplex Virus Type 1 Infection by Silver Nanoparticles Capped with Mercaptoethane Sulfonate
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DOI：
10.1021/bc900215b
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发表时间：
2009-08-01
影响因子：
4.7
通讯作者：
Sarid, Ronit
中科院分区：
文献类型：
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作者：
Baram-Pinto, Dana;Shukla, Sourabh;Sarid, Ronit
生物分子和纳米颗粒之间的相互作用表明纳米颗粒可用于各种医疗干预。单纯疱疹病毒1型(HSV-1)的附着和进入细胞涉及病毒包膜糖蛋白和细胞表面硫酸肝素(HS)之间的相互作用。基于这一机理，我们设计了以硫代乙烷磺酸(Ag-MES)为封端的银纳米粒子。预计这些纳米粒子将针对病毒，并通过其磺酸盐端基竞争其与细胞HS的结合，从而阻止病毒进入细胞并防止后续感染。采用超声化学方法合成了结构明确、易在水中再分散的银-MES纳米粒子。未观察到这些纳米颗粒对宿主细胞的毒性作用。包被的纳米粒子在细胞培养中有效地抑制了HSV-1的感染。然而，可溶性表面活性物质MES的应用并不能抑制病毒感染，这意味着银-MES纳米粒子的抗病毒作用是由其多价性和表面空间定向MES所赋予的。我们的结果表明，被封顶的纳米颗粒可以作为有用的局部药物，用于预防依赖石油HS进入的病原体感染。
Interactions between biomolecules and nanoparticles suggest the use of nanoparticles for various medical interventions. The attachment and entry of herpes simplex virus type 1 (HSV-1) into cells involve interaction between viral envelope glycoproteins and cell surface heparan sulfate (HS). Based oil this mechanism, we designed silver nanoparticles that are capped with mercaptoethane sulfonate (Ag-MES). These nanoparticles are predicted to target the virus and to compete for its binding to cellular HS through their sulfonate end groups, leading to the blockage of viral entry into the cell and to the prevention of subsequent infection. Structurally defined Ag-MES nanoparticles that are readily redispersible in water were sonochemically synthesized. No toxic effects of these nanoparticles on host cells were observed. Effective inhibition of HSV-1 infection in Cell Culture by the capped nanoparticles was demonstrated. However, application of the soluble surfactant MES failed to inhibit viral infection, implying that the antiviral effect of Ag-MES nanoparticles is imparted by their multivalent nature and spatially directed MES on the surface. Our results suggest that capped nanoparticles may serve as useful topical agents for the prevention of infections with pathogens dependent oil HS for entry.