Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement.
Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement.
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使用大肠杆菌内膜作为支架来锚定酶以增强代谢通量
DOI：
10.1002/elsc.202200034
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发表时间：
2023-02
影响因子：
2.7
通讯作者：
Ma, Gang
中科院分区：
文献类型：
--
作者：
Wang, You;Wang, Yushu;Wu, Yuqi;Suo, Yang;Guo, Huaqing;Yu, Yineng;Yin, Ruonan;Xi, Rui;Wu, Jiajie;Hua, Nan;Zhang, Yuehan;Zhang, Shaobo;Jin, Zhenming;He, Lin;Ma, Gang
在同一代谢途径中聚集酶是提高生产力的自然策略。合成蛋白质、RNA和DNA支架已经被设计成人工聚集细胞中的多种酶，这需要复杂的构建过程，并且具有有限的靶酶槽。利用大肠杆菌内细胞膜作为天然支架，将四种脂肪酸脱氢酶（FAS）聚簇在一起，实现了体内脂肪酸合成效率的提高。构建策略简单到将靶酶融合到膜锚蛋白（Lgt）的N末端或C末端，并且锚定酶的数量不受限制。这种新型装置不仅在聚集多个酶方面表现出与其他人工支架相似的效率，而且还促进产物分泌，驱动整个代谢通量向前，并与细胞质支架系统相比进一步提高了总产率。
Clustering enzymes in the same metabolic pathway is a natural strategy to enhance productivity. Synthetic protein, RNA and DNA scaffolds have been designed to artificially cluster multiple enzymes in the cell, which require complex construction processes and possess limited slots for target enzymes. We utilized the Escherichia coli inner cell membrane as a native scaffold to cluster four fatty acid synthases (FAS) and achieved to improve the efficiency of fatty acid synthesis in vivo. The construction strategy is as simple as fusing target enzymes to the N‐terminus or C‐terminus of the membrane anchor protein (Lgt), and the number of anchored enzymes is not restricted. This novel device not only presents a similar efficiency in clustering multiple enzymes to that of other artificial scaffolds but also promotes the product secretion, driving the entire metabolic flux forward and further increasing the gross yield compared with that in a cytoplasmic scaffold system.
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影响因子：
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