Induction of coordinated movement of Myxococcus xanthus cells
Induction of coordinated movement of Myxococcus xanthus cells
复制标题
黄色粘球菌细胞协调运动的诱导
DOI：
10.1128/jb.152.1.451-461.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
D. Kaiser
中科院分区：
文献类型：
--
作者：
L. Shimkets;D. Kaiser
有节奏地前进的细胞波，称为涟漪，在黄色粘球菌聚集成子实体的过程中自发产生。通过洗涤波纹细胞制备的提取物含有一种能诱导静止细胞产生波纹的物质。三条证据表明，胞壁蛋白（肽聚糖）是提取物中的波纹诱导物质。首先，波纹诱导活性与超声破坏的M. xanthus细胞第二，来自各种不同细菌的完整细胞、细胞提取物或纯化的胞壁蛋白都能够诱导涟漪。相比之下，从甲烷杆菌属制备的提取物。其含有假胞壁素而不是典型的细菌胞壁素，不能诱导波纹。第三，M. xanthusmurein、N-乙酰葡糖胺、N-乙酰胞壁酸、二氨基庚二酸和D-丙氨酸能够诱导波纹。聚集细胞产生的涟漪的波长为45微米，最大速度为2微米/分钟。控制滑行运动的多基因系统似乎都需要涟漪，所有已知的突变在spoC位点消除涟漪和孢子形成。
Rhythmically advancing waves of cells, called ripples, arise spontaneously during the aggregation of Myxococcus xanthus into fruiting bodies. Extracts prepared by washing rippling cells contain a substance that will induce quiescent cells to ripple. Three lines of evidence indicate that murein (peptidoglycan) is the ripple-inducing substance in the extracts. First, ripple-inducing activity is associated with the cell envelope of sonically disrupted M. xanthus cells. Second, whole cells, cell extracts, or purified murein from a variety of different bacteria are capable of inducing ripples. In contrast, extracts prepared from Methanobacterium spp. which contain pseudomurein instead of typical bacterial murein fail to induce ripples. Third, four components of M. xanthus murein, N-acetylglucosamine, N-acetylmuramic acid, diaminopimelate, and D-alanine, are able to induce ripples. Ripples produced by aggregating cells have a wavelength of 45 micrometers and a maximum velocity of 2 micrometers/min. Both of the multigene systems that control gliding motility appear to be required for rippling, and all known mutations at the spoC locus eliminate both rippling and sporulation.