Gut Microbiome Associates With Lifetime Cardiovascular Disease Risk Profile Among Bogalusa Heart Study Participants.
Gut Microbiome Associates With Lifetime Cardiovascular Disease Risk Profile Among Bogalusa Heart Study Participants.
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DOI：
10.1161/circresaha.116.309219
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发表时间：
2016-09-30
影响因子：
20.1
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Kelly TN;Bazzano LA;Ajami NJ;He H;Zhao J;Petrosino JF;Correa A;He J
很少有研究系统地评估肠道微生物群对心血管疾病(CVD)风险的影响。在55名Bogalusa心脏研究(BHS)参与者中，研究肠道微生物区系与终生CVD风险概况之间的关系。对BHS参与者粪便中提取的微生物DNA进行16S rRNA测序。阿尔法多样性，包括丰富度和均衡度的衡量标准，以及个体属与终生心血管疾病风险概况的相关性进行了测试。多变量回归技术被用来调整年龄、性别和种族(模型1)，以及身体质量指数(BMI)(模型2)和BMI和饮食(模型3)。在模型1中，丰富度每个标准差增加的优势比(95%可信区间)分别为0.62(0.39，0.99)、0.61(0.38，0.98)和0.63(0.39，0.99)。关联关系在模型2和模型3中是一致的。在所有模型中，心血管疾病风险分布高与低的患者中有四个属被丰富。模型1的p值分别为：普氏原虫2.12×10−3、普氏原虫7.95×10−5、普氏原虫4.39×10−4、普氏原虫1.5 1×10−4。在所有模型中，心血管疾病风险分布高与低的患者中有两个属被耗尽。模型1的P值分别为：别杆菌2.96×10−6，链杆菌1.82×10−4。目前的研究确定了总体微生物丰富度和六个微生物属与终生心血管疾病风险的关联。
Few studies have systematically assessed the influence of gut microbiota on cardiovascular disease (CVD) risk. To examine the association between gut microbiota and lifetime CVD risk profile among 55 Bogalusa Heart Study (BHS) participants with the highest and 57 with the lowest lifetime burdens of CVD risk factors. 16S rRNA sequencing was conducted on microbial DNA extracted from stool samples of the BHS participants. Alpha diversity, including measures of richness and evenness, and individual genera were tested for associations with lifetime CVD risk profile. Multivariable regression techniques were employed to adjust for age, gender, and race (Model 1), along with body mass index (BMI) (Model 2) and both BMI and diet (Model 3). In Model 1, odds ratios (95% confidence intervals) for each standard deviation increase in richness, measured by the number of observed operational taxonomic units, Chao 1 index, and abundance-based coverage estimator, were 0.62 (0.39, 0.99), 0.61 (0.38, 0.98), and 0.63 (0.39, 0.99), respectively. Associations were consistent in Models 2 and 3. Four genera were enriched among those with high versus low CVD risk profile in all models. Model 1 p-values were: 2.12×10−3, 7.95×10−5, 4.39×10−4, and 1.51×10−4 for Prevotella 2, Prevotella 7, Tyzzerella and Tyzzerella 4, respectively. Two genera were depleted among those with high versus low CVD risk profile in all models. Model 1 P-values were: 2.96×10−6 and 1.82×10−4 for Alloprevotella and Catenibacterium, respectively. The current study identified associations of overall microbial richness and six microbial genera with lifetime CVD risk.