Can coelenterates make coelenterazine? Dietary requirement for luciferin in cnidarian bioluminescence
Can coelenterates make coelenterazine? Dietary requirement for luciferin in cnidarian bioluminescence
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DOI：
10.1073/pnas.201329798
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发表时间：
2001-09-25
影响因子：
11.1
通讯作者：
Robison, BH
中科院分区：
文献类型：
--
作者：
Haddock, SHD;Rivers, TJ;Robison, BH
在钙激活的发光蛋白水母发光蛋白中，光是由腔肠素氧化产生的，腔肠素是至少七种海洋门使用的发光蛋白。然而，尽管对发光蛋白进行了广泛的研究，但没有证据表明腔肠素起源于刺胞动物门。在这里，我们报告说，水螅水母Aequorea维多利亚是无法产生自己的腔肠素，是依赖于饮食供应的这种生物发光的荧光素。虽然它们含有功能性的脱辅基蛋白，但在无维生素饮食中饲养的水母不能产生光，除非从外部来源提供腔肠素。这一证据有关的起源刺胞动物的生物发光的进化和广泛使用的腔肠素之间的海洋生物的影响。
In the calcium-activated photoprotein aequorin, light is produced by the oxidation of coelenterazine, the luciferin used by at least seven marine phyla. However, despite extensive research on photoproteins, there has been no evidence to indicate the origin of coelenterazine within the phylum Cnidaria. Here we report that the hydromedusa Aequorea victoria is unable to produce its own coelenterazine and is dependent on a dietary supply of this luciferin for bioluminescence. Although they contain functional apophotoproteins, medusae reared on a luciferin-free diet are unable to produce light unless provided with coelenterazine from an external source. This evidence regarding the origins of luciferin in Cnidaria has implications for the evolution of bioluminescence and for the extensive use of coelenterazine among marine organisms.