Direct delivery and submicrometer Patterning of DNA by a nanofountain probe
Direct delivery and submicrometer Patterning of DNA by a nanofountain probe
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DOI：
10.1002/adma.200701254
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发表时间：
2008-01-18
影响因子：
29.4
通讯作者：
Espinosa, Horacio D.
中科院分区：
文献类型：
--
作者：
Kim, Keun-Ho;Sanedrin, Raymond G.;Espinosa, Horacio D.
生物分子图案化是使用纳米喷泉探头(NFP)实现的，它将DNA的液体溶液通过火山状的孔传递到底物(见图)。直径小至200纳米的斑点特征通常可以在室温下在较宽的湿度范围内制作。与图案DNA互补的序列功能化的金纳米颗粒表明，沉积的特征保持了生物活性。
Biomolecular patterning is achieved using a nanofountain probe (NFP) that delivers a liquid solution of DNA through a volcano-like aperture to a substrate (see figure). Spot features as small as 200 nm in diameter can be routinely made at room temperature over a wide humidity range. Gold nanoparticles functionalized with sequences complementary to the patterned DNA demonstrate that biological activity is retained by the deposited features.