Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions
Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions
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DOI：
10.1016/j.bbrc.2006.11.088
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发表时间：
2007-01-19
影响因子：
3.1
通讯作者：
Tsumoto, Kouhei
中科院分区：
文献类型：
--
作者：
Nagatoishi, Satoru;Tanaka, Yoshikazu;Tsumoto, Kouhei
值得注意的是，DNA G-四链体的形成是由稳定阳离子以外的因素诱导的，因为这一事件可能发生在活细胞中。以往的研究表明，凝血酶结合DNA适体（TBA）形成一个椅子型的分子内G-四链体结构，在没有稳定阳离子的情况下与凝血酶蛋白结合。在这里，我们使用圆二色性（CD）光谱，以确认G-四链体的形成在凝血酶的存在下，没有稳定的阳离子。我们得到的特征CD光谱表明，TBA形成独特的G-四链体结构。此外，我们研究了G-四链体的形成诱导的溶剂环境的变化：低温条件和分子拥挤的影响。(c)2006年爱思唯尔公司All rights reserved.
It is noteworthy that the formation of the DNA G-quadruplex is induced by factors other than stabilizing cations because this event probably occurs in living cells. Previous studies have shown that thrombin-binding DNA aptamer (TBA) forms a chair-type intramolecular G-quadruplex structure that binds with thrombin protein in the absence of stabilizing cations. Here, we used circular dichroism (CD) spectroscopy to confirm G-quadruplex formation in the presence of thrombin without stabilizing cations. We obtained characteristic CD spectra that demonstrated that TBA forms the distinctive G-quadruplex structure. Additionally, we investigated G-quadruplex formation induced by change of solvent environment: the influence of low-temperature conditions and molecular crowding. (c) 2006 Elsevier Inc. All rights reserved.