Effect of Oligosaccharide Lewis A Mimic Peptide on Acute Peritoneal Inflammation in Mice
Effect of Oligosaccharide Lewis A Mimic Peptide on Acute Peritoneal Inflammation in Mice
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发表时间：
2005
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通讯作者：
L. Qing
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作者：
L. Qing
选择低聚糖刘易斯A模拟肽作为特异性抑制剂，通过抑制E-选择素与其配体的结合来抑制炎症反应。将表达寡糖刘易斯A模拟肽IELLQAR或合成肽IELLQAR的小鼠分别注射到小鼠尾静脉中，注射Zymogan 3 h后，对照组为不表达IELLQAR或BSA的小鼠。与对照实验相比，在鼠体内急性炎症模型中施用噬菌体展示肽或合成肽后，观察到进入腹腔的嗜中性粒细胞募集的统计学显著减少。为了证实这些结果，MPO活性通过荧光光度法测量为从腹膜腔收集的细胞匀浆中的吸光度率。观察到的酶活性降低与通过总中性粒细胞计数评估的中性粒细胞数量减少平行。结合上述结果，注射寡糖刘易斯A模拟肽IELLQAR可以通过阻止E-选择素与其配体结合来抑制急性炎症。
Oligosaccharide Lewis A mimic peptide as specific inhibitor was selected to investigate the possibility of the inhibition of inflammation by preventing E-selectin from binding to its ligand.The mice were injected intraperitoneally(i_p) with single-dose Zymosan. The phages expressing oligosaccharide Lewis A mimic peptide IELLQAR or synthetic peptide IELLQAR were separately injected into the mice tail vein after injection of Zymogan for 3 h, and the control was phages not expressing IELLQAR or BSA. Compared with control experiments, the statistically significant reduction of neutrophil recruitment entering into the intraperitoneal cavity was observed upon administration of the phage-displaying peptide or synthetic peptide in a murine acute inflammation model in vivo. To confirm these results, MPO activity was measured spectrophotometrically as an absorbance rate in the homogenates of cells collected from the peritoneal cavity. The reduction of enzymatic activity observed was in parallel with the decrease of neutrophil numbers assessed by total neutrophil count. Together with above results, the injection of oligosaccharide Lewis A mimic peptide IELLQAR can inhibit acute inflammation by preventing E-selectin from binding to its ligand.