NA+-DEPENDENT, ALPHA-ADRENERGIC MOBILIZATION OF INTRACELLULAR (MITOCHONDRIAL) CA-2+ IN BROWN ADIPOCYTES
NA+-DEPENDENT, ALPHA-ADRENERGIC MOBILIZATION OF INTRACELLULAR (MITOCHONDRIAL) CA-2+ IN BROWN ADIPOCYTES
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DOI：
10.1111/j.1432-1033.1984.tb08173.x
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
NEDERGAARD, J
中科院分区：
文献类型：
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作者：
CONNOLLY, E;NANBERG, E;NEDERGAARD, J
用~（45）Ca ~（2+）标记技术研究了金黄地鼠棕色脂肪细胞内存在一个对钙离子敏感的、可快速动员的Ca ~（2+）池及其意义。这样一个池是存在的，可能位于丰富的线粒体。它通过α-去甲肾上腺素（中值有效浓度50 nM）快速动员。肾上腺素能机制Ca 2+从细胞内储存（线粒体）的动员需要细胞外Na+的存在，但不需要Ca 2+、K+或Mg 2+。显然，该实验与将细胞内Ca 2+库的动员与α-Ca 2+结合的假设一致。肾上腺素能诱导的质膜Na+渗透性的增加（观察为膜去极化），以及随后的线粒体Na+/Ca 2+交换的激活，导致线粒体Ca 2+的动员和α-Ca 2+的介导。肾上腺素能效应是由于细胞溶质Ca 2+水平升高。
The existence and significance of a hormone-sensitive, rapidly mobilizable intracellular pool of Ca2+ in hamster brown-fat cells was investigated with 45Ca2+-labeling techniques. Such a pool existed and was probably located within the abundant mitochondria. It was rapidly mobilized by norepinephrine (median effective concentration 50 nM) through .alpha.-adrenergic mechanisms. The mobilization of Ca2+ from the intracellular stores (mitochondria) required the presence of extracellular Na+, but not of Ca2+, K+ or Mg2+. Evidently, the experiments are in agreement with a hypothesis linking the mobilization of intracellular Ca2+ pools with an .alpha.-adrenergically-induced increase in plasma membrane Na+ permeability (observed as a membrane depolarization), and a subsequent activation of the mitochondrial Na+/Ca2+ exchange, leading to mobilization of mitochondrial Ca2+ and the mediation of .alpha.-adrenergic effects as a result of an elevated cytosolic Ca2+ level.