Effects of inefficient cleavage of the signal sequence of HIV-1 gp120 on its association with calnexin, folding, and intracellular transport
Effects of inefficient cleavage of the signal sequence of HIV-1 gp120 on its association with calnexin, folding, and intracellular transport
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DOI：
10.1073/pnas.93.18.9606
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发表时间：
1996-09-03
影响因子：
11.1
通讯作者：
Kang, CY
中科院分区：
文献类型：
--
作者：
Li, Y;Bergeron, JJJM;Kang, CY
HIV-1包膜糖蛋白gp 120表现出低效的细胞内转运，这是由于其滞留在内质网中所致。在昆虫细胞（Sig）的HIV-1 gp 120与钙连接蛋白的共表达表明，它们的相互作用是由HIV-1 gp 120的信号序列调节。具有天然信号序列的gp 120与钙连接蛋白的结合时间延长，t1/2大于20 min。用来自蜂毒素的信号序列替换天然信号序列导致gp 120与钙连接蛋白结合时间缩短（t1/2
The HIV-1 envelope glycoprotein gp120 displays inefficient intracellular transport, which is caused by its retention in the endoplasmic reticulum. Coexpression in insect cells (Sig) of HIV-1 gp120 with calnexin has shown that their interaction was modulated by the signal sequence of HIV-1 gp120. gp120, with its natural signal sequence, showed a prolonged association with calnexin with a t1/2 of greater than 20 min, Replacement of the natural signal sequence with the signal sequence from mellitin led to a decreased time of association of gp120 with calnexin (t1/2