Identification of isethionic acid and other small molecule metabolites of Fragilariopsis cylindrus with nuclear magnetic resonance
Identification of isethionic acid and other small molecule metabolites of Fragilariopsis cylindrus with nuclear magnetic resonance
复制标题
核磁共振鉴定圆柱脆杆藻羟乙磺酸等小分子代谢物
DOI：
10.1007/s00216-012-6169-2
复制
发表时间：
2012-08-01
影响因子：
4.3
通讯作者：
Bearden, Daniel W.
中科院分区：
文献类型：
--
作者：
Boroujerdi, Arezue F. B.;Lee, Peter A.;Bearden, Daniel W.
核磁共振（NMR）光谱已被用来获得代谢配置文件的极性硅藻Fragilariopsis cylindrus，导致在这种生物体中的一种新的代谢产物的鉴定。一项正在进行的代谢组学研究的初步结果发现，羟乙基磺酸（2-羟基乙磺酸，CAS：107-36-8）是F.圆柱形。该化合物由生物体在正常培养条件下产生。这一发现是硅藻产生羟乙磺酸的首次报道。除羟乙基磺酸外，还有四种代谢产物，二甲基磺基丙酸酯（DMSP）、甜菜碱、甲根碱和脯氨酸，它们可能在F.圆柱形。基于NMR的F.沿着生物体的生长曲线沿着获得圆柱体。在一个生长期内监测了5种代谢物的相对浓度水平。18 ~ 25天为圆柱形。除脯氨酸在第21天后开始下降外，所有样品的相对浓度均随时间增加。
Nuclear magnetic resonance (NMR) spectroscopy has been used to obtain metabolic profiles of the polar diatom Fragilariopsis cylindrus, leading to the identification of a novel metabolite in this organism. Initial results from an ongoing metabolomics study have led to the discovery of isethionic acid (2-hydroxyethanesulfonic acid, CAS: 107-36-8) as a major metabolite in F. cylindrus. This compound is being produced by the organism under normal culture conditions. This finding is the first report of a diatom producing isethionic acid. In addition to isethionic acid, four other metabolites, dimethylsulfoniopropionate (DMSP), betaine, homarine, and proline were present and may serve as osmoprotectants in F. cylindrus. NMR-based metabolite profiles of F. cylindrus were obtained along a growth curve of the organism. The relative concentration levels of the five metabolites were monitored over a growth period of F. cylindrus from 18 to 25 days. All showed an increase in relative concentration with time, except for proline, which began to decrease after day 21.