Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genes
Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genes
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DOI：
10.1111/1462-2920.14560
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发表时间：
2019-05-01
影响因子：
5.1
通讯作者：
Moran, Mary Ann
中科院分区：
文献类型：
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作者：
Nowinski, Brent;Motard-Cote, Jessie;Moran, Mary Ann
二甲基磺基丙酸盐（DMSP）是一种丰富的有机硫代谢产物，由许多浮游植物产生，并通过两种不同的途径被细菌降解，具有气候相关的影响。我们评估了2014年9月至10月在加利福尼亚州蒙特雷湾3周时间内浮游植物群落组成背景下拥有这些途径的细菌的多样性和丰度。来自DMSP去甲基化途径的dmdA基因在DMSP基因库中占主导地位，主要由α-变形菌SAR 11进化枝的成员携带，其次是由Roseflora组携带，特别是在研究的后半部分。DMSP降解社区的新成员出现了从宏基因组组装和单细胞测序，包括很大程度上未表征的gammaproteobacteria和alphaproteobacteria类群恢复的dmdA序列。在DMSP裂解途径中，SAR 11基因dddK在研究早期是最丰富的，但随着时间的推移被dddP取代。SAR 11成员，特别是那些窝藏的DMSP降解途径的基因，有一个很强的正相关的甲藻的丰度，和DMSP降解gammaproteobacteria共发生与haptophytes。对沿海生态系统中DMSP命运驱动因素的原位研究首次证明了特定细菌DMSP降解物和浮游植物类群之间的相关性。
Dimethylsulfoniopropionate (DMSP) is an abundant organic sulfur metabolite produced by many phytoplankton species and degraded by bacteria via two distinct pathways with climate-relevant implications. We assessed the diversity and abundance of bacteria possessing these pathways in the context of phytoplankton community composition over a 3-week time period spanning September-October, 2014 in Monterey Bay, CA. The dmdA gene from the DMSP demethylation pathway dominated the DMSP gene pool and was harboured mostly by members of the alphaproteobacterial SAR11 clade and secondarily by the Roseobacter group, particularly during the second half of the study. Novel members of the DMSP-degrading community emerged from dmdA sequences recovered from metagenome assemblies and single-cell sequencing, including largely uncharacterized gammaproteobacteria and alphaproteobacteria taxa. In the DMSP cleavage pathway, the SAR11 gene dddK was the most abundant early in the study, but was supplanted by dddP over time. SAR11 members, especially those harbouring genes for both DMSP degradation pathways, had a strong positive relationship with the abundance of dinoflagellates, and DMSP-degrading gammaproteobacteria co-occurred with haptophytes. This in situ study of the drivers of DMSP fate in a coastal ecosystem demonstrates for the first time correlations between specific groups of bacterial DMSP degraders and phytoplankton taxa.