The Phospholipid Composition of Kangaroo Spermatozoa Verified by Mass Spectrometric Lipid Analysis
The Phospholipid Composition of Kangaroo Spermatozoa Verified by Mass Spectrometric Lipid Analysis
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DOI：
10.1007/s11745-017-4283-9
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发表时间：
2017-10-01
期刊：
影响因子：
1.9
通讯作者：
Jakop, Ulrike
中科院分区：
文献类型：
--
作者：
Engel, Kathrin M.;Schiller, Juergen;Jakop, Ulrike
迄今为止，袋鼠精子的冷冻保存尚未成功，但这些细胞的冷冻保存方案也没有前景。然而，配子的保护是极其重要的，特别是在保护濒危物种的背景下。由于精子由不同的膜系统组成，这些膜的组成可能是冷冻保存困难的原因。脂质作为影响生物膜物理特性的主要成分，在精子成熟过程中起着重要作用。因此，脂质组成的知识是至关重要的任何进一步的步骤，以保护物种。采用MALDI-TOF、ESI-IT、串联质谱和薄层色谱法对4种不同袋鼠附睾精子的脂质组成进行了研究。这些物种的光谱与所鉴定的脂质种类非常相似。在精子从附睾头到附睾尾的运输过程中，脂质组成发生了巨大的变化，特别是多不饱和脂肪酸、醚类脂质和磷脂原的增加，以及单和双不饱和脂肪酸的减少。此外，含有二十二碳三烯酸（22:3）的磷脂酰胆碱在袋鼠精子中含量最高，这是一种迄今为止未知的用于精子膜的脂肪酸。
Cryopreservation of kangaroo sperm has not been successful so far, and yet there is no promising cryopreservation protocol for these cells available. However, conservation of gametes is extremely important, particularly in the context of preserving endangered species. As spermatozoa are comprised of different membrane systems, the composition of these membranes might account for difficulties in cryopreservation. Lipids, as the main components, affect the physical properties of biological membranes and play a major role in sperm maturation. Therefore, knowledge of the lipid composition is crucial for any further step toward the preservation of the species. We used MALDI-TOF, ESI-IT, tandem mass spectrometry, and thin layer chromatography to investigate the lipid composition of epididymal spermatozoa of four different kangaroo species. Spectra of these species were very similar with respect to the identified lipid species. Tremendous changes in the lipid composition during the transit of sperm from caput to cauda epididymis could be seen, specifically an increase in poly-unsaturated fatty acids, ether lipids, and plasmalogens, as well as a reduction in mono- and di-unsaturated fatty acids. Additionally, phosphatidylcholines containing docosatrienoic acid (22:3), a heretofore unknown fatty acid for sperm membranes, showed the highest abundance in kangaroo sperm.