High-resolution proton nuclear magnetic resonance studies of the exchangeable resonances of the lac repressor headpiece.
High-resolution proton nuclear magnetic resonance studies of the exchangeable resonances of the lac repressor headpiece.
复制标题
紫胶抑制器头件可交换共振的高分辨率质子核磁共振研究。
DOI：
10.1021/bi00515a006
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Jardetzky,O
中科院分区：
文献类型：
--
作者：
Wemmer,D;Shvo,H;Ribeiro,AA;Bray,RP;Jardetzky,O
D. Wemmer，H. Shvo，AA Ribeiro，R. P. Bray和O. Jardetzky* 摘要：用高分辨率NMR研究了乳糖阻遏物N-末端51个氨基酸头部（HP）的可交换氢。在p2 H 4.2的2 H2O溶液中可观察到来自35±3个质子的共振。其中一些的交换物已经在p2 H范围1.5-5内测量。在H2O中，在pH 3.2下可观察到来自70±6个质子的共振，在pH 7.1下可观察到来自35±5个质子的共振。自从Geisler和Weber（1977）表明可以在氨基酸51或59处选择性地切割lac阻遏物，并且较小的片段能够结合脱氧核糖核酸（DNA）以来，人们对确定该片段的结构及其与DNA相互作用的性质产生了相当大的兴趣[关于迄今为止的研究讨论，参见Ribeiro et al.（1981年b）]。可交换羟基的NMR研究
D. Wemmer, H. Shvo, AA Ribeiro, R. P. Bray, and O. Jardetzky* abstract: The exchangeable hydrogens of the N-terminal 51 amino acid headpiece (HP) of the lac repressor have been examined with high-resolution NMR. Resonances from 35±3 protons are observable in 2H20 solutions at p2H 4.2. The exchangerates for some of these have been measured over the p2H range 1.5-5. In H20, resonances from 70±6 protons are observable at pH 3.2 and 35±5 protons at pH 7.1. TheSince Geisler & Weber (1977) showed that it was possible to selectively cleave the lac repressor at amino acid 51 or 59 and that the smaller fragment is ableto bind deoxyribonucleic acid (DNA), there has been considerable interest in deter-mining the structure of this fragment and the nature of its interaction with DNA [for a discussion of studies to date, see Ribeiro et al.(1981b)]. NMR studies of exchangeable hy-