Differential Expression of IL-36 Family Members and IL-38 by Immune and Nonimmune Cells in Patients with Active Inflammatory Bowel Disease
Differential Expression of IL-36 Family Members and IL-38 by Immune and Nonimmune Cells in Patients with Active Inflammatory Bowel Disease
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DOI：
10.1155/2018/5140691
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Yamamoto-Furusho, Jesus K.
中科院分区：
文献类型：
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作者：
Fonseca-Camarillo, Gabriela;Furuzawa-Carballeda, Janette;Yamamoto-Furusho, Jesus K.
白细胞介素 - 1（IL - 1）家族包括白细胞介素 - 38（IL - 1F10）以及白细胞介素 - 36亚家族，是炎症性疾病的核心介质，这些疾病包括脓疱型银屑病、特应性皮炎、类风湿性关节炎和肠道炎症。本研究的目的是评估炎症性肠病（IBD）患者组织中白细胞介素 - 36α、白细胞介素 - 36β、白细胞介素 - 36γ、白细胞介素 - 36受体拮抗剂（IL - 36Ra）以及白细胞介素 - 38的基因和细胞表达及其与临床活动的相关性。
患者和方法：进行了一项横断面比较研究。纳入70例IBD患者和30例无炎症的非IBD对照者。通过逆转录聚合酶链反应（RT - PCR）测量基因表达。通过双重染色免疫组化检测蛋白质表达。
结果：与克罗恩病组和非炎症对照组相比，活动期溃疡性结肠炎患者结肠黏膜中白细胞介素 - 36家族成员的信使核糖核酸（mRNA）表达增加，但白细胞介素 - 38的表达未增加（P
IL-1 family includes IL-38 (IL-1F10) and the subfamily of IL-36 and is the central mediators of inflammatory diseases, including pustular psoriasis, atopic dermatitis, rheumatoid arthritis, and gut inflammation. The purpose of the study was to evaluate on tissue of the patients with inflammatory bowel disease (IBD), the IL-36 alpha, IL-36 beta, IL-36 gamma, IL-36Ra, and IL-38 gene and cell expression and its correlation with clinical activity. Patients and Methods. A cross-sectional and comparative study was performed. Seventy patients with IBD and 30 noninflamed non-IBD controls were enrolled. Gene expression was measured by RT-PCR. Protein expression was detected by double-staining immunohistochemistry. Results. The mRNA expression of IL-36 family members but not IL-38 was increased in colonic mucosa from patients with active ulcerative colitis versus Crohn's disease group and noninflammatory control group (P