A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene
A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene
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DOI：
10.1093/emboj/17.19.5718
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发表时间：
1998-10-01
期刊：
影响因子：
11.4
通讯作者：
de Crombrugghe, B
中科院分区：
文献类型：
--
作者：
Lefebvre, V;Li, P;de Crombrugghe, B
一种新形式的Sox 5（称为L-Sox 5）和Sox 6的转录本与Sox 9在小鼠胚胎的所有软骨形成部位共表达。卷曲螺旋结构域位于L-Sox 5的N-末端部分，并且在Sox 5中不存在，与Sox 6中的类似结构域显示>90%的同一性，并且介导与Sox 6的同源二聚化和异源二聚化。L-Sox 5/Sox 6的二聚化大大增加了两种Sox蛋白与含有相邻HMG位点的DNA的结合效率。L-Sox 5、Sox 6和Sox 9协同激活10 T1/2和MC 615细胞中软骨细胞分化标志物Col 2a 1的表达。该基因中的一个48 bp的软骨细胞特异性增强子含有增强子活性所必需的几个HMG样位点，结合三种Sox蛋白，并在非软骨细胞中被三种Sox蛋白协同激活。我们的数据表明，L-Sox 5/Sox 6和Sox 9，这属于两个不同类别的Sox转录因子，相互合作的表达Col 2a 1和可能的其他基因的软骨细胞程序。
Transcripts for a new form of Sox5, called L-Sox5, and Sox6 are coexpressed with Sox9 in all chondrogenic sites of mouse embryos. A coiled-coil domain located in the N-terminal part of L-Sox5, and absent in Sox5, showed >90% identity with a similar domain in Sox6 and mediated homodimerization and heterodimerization with Sox6. Dimerization of L-Sox5/Sox6 greatly increased efficiency of binding of the two Sox proteins to DNA containing adjacent HMG sites. L-Sox5, Sox6 and Sox9 cooperatively activated expression of the chondrocyte differentiation marker Col2a1 in 10T1/2 and MC615 cells, A 48 bp chondrocyte-specific enhancer in this gene, which contains several HMG-like sites that are necessary for enhancer activity, bound the three Sox proteins and was cooperatively activated by the three Sox proteins in non-chondrogenic cells. Our data suggest that L-Sox5/Sox6 and Sox9, which belong to two different classes of Sox transcription factors, cooperate with each other in expression of Col2a1 and possibly other genes of the chondrocytic program.