Beta interferon inhibits Toxoplasma gondii growth in human monocyte-derived macrophages
Beta interferon inhibits Toxoplasma gondii growth in human monocyte-derived macrophages
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DOI：
10.1128/iai.57.10.3254-3256.1989
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发表时间：
1989-10
影响因子：
3.1
通讯作者：
J L Schmitz;J M Carlin;E C Borden;G I Byrne
中科院分区：
文献类型：
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作者：
J L Schmitz;J M Carlin;E C Borden;G I Byrne
γ干扰素（IFN-γ）以前已被证明可以通过诱导吲哚胺2，3-双加氧酶（IDO）活性来阻断弓形虫在成纤维细胞中的复制。IFN-β也已知在单核细胞衍生的巨噬细胞中诱导IDO活性，但其阻断T.弓形虫病尚未得到证实。我们发现，IFN-β和脂多糖的组合不仅比单独IFN-β在单核细胞衍生的巨噬细胞中诱导更大的IDO活性，而且这种组合也有效抑制T.刚地。此外，添加外源性色氨酸可以逆转这种抑制作用，从而证明了IFN-β抑制T. IDO诱导弓形虫复制。
Gamma interferon (IFN-gamma) previously has been shown to block the replication of Toxoplasma gondii in fibroblasts by the induction of indoleamine 2,3-dioxygenase (IDO) activity. IFN-beta also is known to induce IDO activity in monocyte-derived macrophages, but its ability to block the growth of T. gondii has not been demonstrated. We found not only that the combination of IFN-beta and lipopolysaccharide induced greater IDO activity in monocyte-derived macrophages than did IFN-beta alone but that this combination also was effective in inhibiting the growth of T. gondii. In addition, the inhibition was reversed by the addition of exogenous tryptophan, thus demonstrating that a mechanism by which IFN-beta inhibited T. gondii replication was by the induction of IDO.