Molecular basis of the dopaminergic system in the cricket Gryllus bimaculatus
Molecular basis of the dopaminergic system in the cricket Gryllus bimaculatus
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DOI：
10.1007/s10158-013-0153-1
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发表时间：
2013-03
影响因子：
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通讯作者：
Takayuki Watanabe;H. Sadamoto;H. Aonuma
中科院分区：
文献类型：
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作者：
Takayuki Watanabe;H. Sadamoto;H. Aonuma
在昆虫中，多巴胺调节行为的各个方面，如学习和记忆，唤醒和运动，也是黑色素的前体。为了阐明蟋蟀Gryllus bimaculatusDeGeer多巴胺能系统的分子基础，我们确定了参与蟋蟀多巴胺生物合成、信号转导和多巴胺再摄取的基因。从蟋蟀脑cDNA文库中分离到酪氨酸羟化酶（TH）的两种异构体的互补DNA。此外，还鉴定了4个多巴胺受体基因（Dop1、Dop2、Dop3和DopEcR）和一个高亲和力多巴胺转运蛋白基因。这两种TH亚型在调节ACT结构域中含有亚型特异性区域，并在不同组织中显示出差异表达模式。此外，多巴胺受体基因具有受体亚型特异性分布：Dop1、Dop2和DopEcR基因在不同组织中广泛表达，表达水平不同，Dop3基因仅在神经组织和睾丸中表达。我们的研究结果提供了一个基本的基础，了解多巴胺的调节不同的生理过程中的板球。
In insects, dopamine modulates various aspects of behavior such as learning and memory, arousal and locomotion, and is also a precursor of melanin. To elucidate the molecular basis of the dopaminergic system in the field cricketGryllus bimaculatusDeGeer, we identified genes involved in dopamine biosynthesis, signal transduction, and dopamine re-uptake in the cricket. Complementary DNA of two isoforms of tyrosine hydroxylase (TH), which convert tyrosine intol-3,4-dihydroxyphenylalanine, was isolated from the cricket brain cDNA library. In addition, four dopamine receptor genes (Dop1,Dop2,Dop3,andDopEcR) and a high-affinity dopamine transporter gene were identified. The two TH isoforms contained isoform-specific regions in the regulatory ACT domain and showed differential expression patterns in different tissues. In addition, the dopamine receptor genes had a receptor subtype-specific distribution: theDop1,Dop2, andDopEcRgenes were broadly expressed in various tissues at differential expression levels, and theDop3gene was restrictedly expressed in neuronal tissues and the testicles. Our findings provide a fundamental basis for understanding the dopaminergic regulation of diverse physiological processes in the cricket.