Dynamic Aspects of Ion Accumulation by Vesicle Traffic Under Salt Stress in Arabidopsis
Dynamic Aspects of Ion Accumulation by Vesicle Traffic Under Salt Stress in Arabidopsis
复制标题
DOI：
10.1093/pcp/pcp143
复制
发表时间：
2009-12-01
影响因子：
4.9
通讯作者：
Mimura, Tetsuro
中科院分区：
文献类型：
--
作者：
Hamaji, Kohei;Nagira, Megumi;Mimura, Tetsuro
研究了拟南芥细胞在高盐胁迫下的细胞膜动态变化。用钠绿色的Na+依赖性荧光观察到，在水培条件下，高浓度NaCl处理拟南芥根尖细胞，液泡体积迅速变化，小的酸室数量减少，小泡运动活跃，Na+在中央液泡和主液泡周围的小泡中积累。详细观察拟南芥悬浮培养细胞在高盐处理下表现出类似的模式，在根尖细胞中观察到的反应。用抗AtNHX 1的抗体对悬浮培养的细胞进行免疫染色，清楚地表明仅在盐处理下细胞质中发生点状荧光。我们还证实了存在AtNHX 1的空泡膜分离悬浮培养的细胞与免疫荧光。空泡Q（a）-SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）蛋白VAM 3/SYP 22的敲除引起盐耐受性的增加。在突变体植物中，Na+在根和芽之间的分布与野生型植物不同，Na+在突变体植物的根中积累更多，而在芽中积累较少。讨论了盐胁迫下囊泡运输的作用。
The intracellular membrane dynamics of Arabidopsis cells under high salt treatment were investigated. When Arabidopsis was treated with high levels of NaCl in hydroponic culture, root tip cells showed rapid changes in the vacuolar volume, a decrease in the number of small acid compartments, active movement of vesicles and accumulation of Na+ both in the central vacuole and in the vesicles around the main vacuole observed with the Na+-dependent fluorescence of Sodium Green. Detailed observation of Arabidopsis suspension-cultured cells under high salt treatment showed a similar pattern of response to that observed in root tip cells. Immunostaining of suspension-cultured cells with antibodies against AtNHX1 clearly showed the occurrence of dotted fluorescence in the cytoplasm only under salt treatment. We also confirmed the existence of AtNHX1 in the vacuolar membrane isolated from suspension-cultured cells with immunofluorescence. Knockout of the vacuolar Q(a)-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein VAM3/SYP22 caused an increase in salt tolerance. In mutant plants, the distribution of Na+ between roots and shoots differed from that of wild-type plants, with Na+ accumulating more in roots and less in the shoots of the mutant plants. The role of vesicle traffic under salt stress is discussed.