Laboratory and field evaluation of three desktop instruments for assay of cholesterol and triglyceride.
Laboratory and field evaluation of three desktop instruments for assay of cholesterol and triglyceride.
复制标题
用于测定胆固醇和甘油三酯的三种台式仪器的实验室和现场评估。
DOI：
10.1093/clinchem/33.7.1230
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发表时间：
1987
影响因子：
9.3
通讯作者：
A. Olsson
中科院分区：
文献类型：
--
作者：
H. von Schenck;L. Treichl;B. Tilling;A. Olsson
我们评估了三种台式仪器，适合将血液胆固醇和甘油三酯的测定分散到卫星和医生的办公室测试。根据其制造商的说明书，使用仪器Ektachem DT 60（E）、Reflotron（R）和Seralyzer（S），在两个医生办公室测定门诊患者的血清或毛细血管血。在中心实验室使用自动离心分析仪（A）评估准确度。用国际质量控制材料监测A的偏倚。我们发现所有的仪器都很容易操作。胆固醇测定的回归方程为：E = 0.92A + 0.7（n = 331，r = 0.94），R（毛细血管血）= 0.96A + 0.3（n = 256，r = 0.95），S = 0.93A + 0.6（n = 260，r = 0.92）。对于甘油三酯，我们获得E = 1.02A（n = 331，r = 0.97），R（cap.血液）= 0.88A（n = 213，r = 0.97），R = 0.94A + 0.1（n = 90，r = 0.99），S = 0.96A（n = 266，r = 0.98）。重复和日内精密度小于8%。日间精密度（一个月内）小于10%。我们强调实验室和现场评估的需要，并强调质量控制的好处。
We evaluated three desktop instruments suitable for decentralizing the assays of blood cholesterol and triglycerides to satellite and physician's office testing. The instruments, Ektachem DT 60 (E), Reflotron (R), and Seralyzer (S), were used according to their manufacturers' instructions to assay serum or capillary blood from outpatients at two physicians offices. Accuracy was assessed in the centralized laboratory by using an automated centrifugal analyzer (A). The bias of A was monitored with an international quality-control material. We found all instruments easy to handle. Regression equations for cholesterol determinations were: E = 0.92A + 0.7 (n = 331, r = 0.94), R (capillary blood) = 0.96A + 0.3 (n = 256, r = 0.95), and S = 0.93A + 0.6 (n = 260, r = 0.92). For triglycerides we obtained E = 1.02A (n = 331, r = 0.97), R (cap. blood) = 0.88A (n = 213, r = 0.97), R = 0.94A + 0.1 (n = 90, r = 0.99), and S = 0.96A (n = 266, r = 0.98). Duplicate and within-day precision was less than 8%. Between-day precision (during a month) was less than 10%. We stress the need of both laboratory and field evaluation and emphasize the benefit of quality control.