Chemical profiling of Houttuynia cordata Thunb. by UPLC-Q-TOF-MS and analysis of its antioxidant activity in C2C12 cells
Chemical profiling of Houttuynia cordata Thunb. by UPLC-Q-TOF-MS and analysis of its antioxidant activity in C2C12 cells
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DOI：
10.1016/j.jpba.2021.114271
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发表时间：
2021-07-26
影响因子：
3.4
通讯作者：
Xiao, Wei
中科院分区：
文献类型：
--
作者：
Ju, Liliang;Zhang, Jiaxing;Xiao, Wei
[医]天仙子鱼腥草是一种传统的中草药，长期以来被用于治疗各种疾病。然而，关于H.缺乏心的水提取物，其对肌肉有益作用的分子基础尚不清楚。为了研究这些问题，在这项研究中，我们使用超高效液相色谱-四极杆-飞行时间-质谱（UPLC-Q-TOF-MS）在正离子和负离子模式下的配置文件，并确定了H的主要成分。鱼腥草水提取物根据质谱和质谱裂解特征，共鉴定出63个峰，包括29个有机酸及其苷类化合物、17个黄酮类化合物、7个挥发性化合物、4个嘧啶和嘌呤衍生物、2个生物碱、2个氨基酸、1个异香草醛和1个香豆素。紫外分光光度法测得总黄酮和多酚含量分别为4.77和139.15 mg/mL。H.用肿瘤坏死因子（TNF）-α处理C2 C12细胞以诱导氧化攻击来评价鱼腥草水提取物。TNF-α诱导的细胞活力下降被逆转处理48小时的提取物，此外，超氧化物歧化酶活性增加，而活性氧水平下降。这些结果为H. cordata提取物，并强调其治疗氧化应激引起的肌肉损伤或疾病的治疗潜力。(c)2021爱思唯尔有限公司版权所有。
Houttuynia cordata Thunb. ("Yu-Xing-Cao"), a traditional Chinese medicinal herb, has long been used to treat various diseases. However, detailed information regarding the chemical constituents of H. cordata aqueous extract is lacking, and the molecular basis of its beneficial effects on muscle is unknown. To investigate these points, in this study, we used ultra-performance liquid chromatography coupled with quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) in positive and negative ion modes to profile and identify the major constituents of H. cordata water extract. A total of 63 peaks were identified based on mass and fragmentation characteristics, including 29 organic acids and their glycosides, 17 flavonoids, 7 volatiles, 4 pyrimidine and purine derivatives, 2 alkaloids, 2 amino acids, 1 isovanillin, and 1 coumarin. The total flavonoid and polyphenol contents of the extract were 4.77 and 139.15 mg/mL, respectively, by ultraviolet spectrophotometry. The cytoprotective activity of H. cordata aqueous extract was evaluated using C2C12 cells treated with tumor necrosis factor (TNF)-a to induce oxidative challenge. The TNF-a induced decrease in cell viability was reversed by treatment for 48 h with the extract; moreover, superoxide dismutase activity was increased while reactive oxygen species level was decreased. These results provide molecular-level evidence for the antioxidant effect of H. cordata extract and highlight its therapeutic potential for the treatment of muscle injury or diseases caused by oxidative stress. (c) 2021 Elsevier B.V. All rights reserved.