Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum
Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum
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DOI：
10.1016/s0167-4889(99)00114-7
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发表时间：
1999-10-13
影响因子：
5.1
通讯作者：
Fujino, M
中科院分区：
文献类型：
--
作者：
Habata, Y;Fujii, R;Fujino, M
利用我们开发的一种寻找孤儿七跨膜结构域受体配体的策略[S。Hinuma等人，Nature 393（1998）272-276]，我们最近鉴定了孤儿7 TMR，APJ的天然配体apelin [K.馆本等人，生物化学、生物物理通信资源251（1998）471-476]。本论文分离了大鼠和小鼠apelin cDNA，并分析了apelin mRNA在大鼠体内的组织分布。虽然apelin mRNA在多种组织中广泛检测到，但在妊娠大鼠的乳腺中检测到apelin mRNA的最高表达。在乳腺中，具有生物活性的Apelin及其mRNA在妊娠期和哺乳期显著增加，并在分娩前后达到最高水平。此外，发现在牛初乳中分泌大量的爱帕琳（14-93 pmol/ml），并且即使在商业牛乳中也仍然可检测到。由于apelin部分抑制小鼠脾细胞响应T细胞受体/CD 3交联的细胞因子产生，因此在初乳和乳汁中口服apelin可能调节新生儿的免疫应答。(C)1999 Elsevier Science B. V.保留所有权利。
By using a strategy that we have developed to search for the ligands of orphan seven-transmembrane-domain receptors [S. Hinuma et al., Nature 393 (1998) 272-276], we have recently identified a natural ligand, apelin, for the orphan 7TMR, APJ [K. Tatemoto et al., Biochem. Biophys. Res. Commun. 251 (1998) 471-476]. In this paper, we isolated rat and mouse apelin cDNAs, and analyzed the tissue distribution of apelin mRNA in rats. Although apelin mRNA was widely detected in a variety of tissues, the highest expression of apelin mRNA was detected in the mammary gland of pregnant rats. In the mammary gland, biologically active apelin and its mRNA considerably increased during pregnancy and lactation, and reached a maximal level around parturition. Moreover, a large amount of apelin (14-93 pmol/ml) was found to be secreted in the bovine colostrum, and it was still detectable even in commercial bovine milk. Since apelin partially suppressed cytokine production by mouse spleen cells in response to T cell receptor/CD3 cross-linking, the oral intake of apelin in the colostrum and milk might modulate immune responses in neonates. (C) 1999 Elsevier Science B.V. All rights reserved.