Non-radioactive and sensitive tracking of neutrophils towards inflammation using antibody functionalized magnetic particle imaging tracers.
Non-radioactive and sensitive tracking of neutrophils towards inflammation using antibody functionalized magnetic particle imaging tracers.
复制标题
DOI：
10.7150/ntno.50721
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Conolly SM
中科院分区：
文献类型：
--
作者：
Chandrasekharan P;Fung KLB;Zhou XY;Cui W;Colson C;Mai D;Jeffris K;Huynh Q;Saayujya C;Kabuli L;Fellows B;Lu Y;Yu E;Tay ZW;Zheng B;Fong L;Conolly SM
白色血细胞（WBC）是哺乳动物免疫系统的关键组成部分，在监视、防御和适应外来病原体方面发挥重要作用。除了它们在主动对抗感染和适应性免疫的发展中的作用外，免疫细胞还参与肿瘤的发展和转移。已经开发了基于抗体的治疗剂来调节（即选择性地激活或抑制免疫功能）和利用免疫细胞来对抗恶性肿瘤。或者，WBC分布的非侵入性跟踪可以诊断炎症、感染、不明原因的发热（FUO）和癌症。磁粒子成像（MPI）是一种非侵入性、非放射性和敏感的医学成像技术，使用安全的超顺磁性氧化铁纳米颗粒（SPIO）作为示踪剂。MPI先前已被证明可以追踪治疗性干细胞超过87天，检测限约为200个细胞。在目前的工作中，我们利用特异于中性粒细胞的抗体缀合的SPIO进行原位标记，并在脂多糖诱导的肌炎的体内小鼠模型中非侵入性和无辐射地追踪这些炎性细胞至感染和炎症部位。MPI显示炎症的灵敏检测，对比噪声比为~8-13。
White blood cells (WBCs) are a key component of the mammalian immune system and play an essential role in surveillance, defense, and adaptation against foreign pathogens. Apart from their roles in the active combat of infection and the development of adaptive immunity, immune cells are also involved in tumor development and metastasis. Antibody-based therapeutics have been developed to regulate (i.e. selectively activate or inhibit immune function) and harness immune cells to fight malignancy. Alternatively, non-invasive tracking of WBC distribution can diagnose inflammation, infection, fevers of unknown origin (FUOs), and cancer. Magnetic Particle Imaging (MPI) is a non-invasive, non-radioactive, and sensitive medical imaging technique that uses safe superparamagnetic iron oxide nanoparticles (SPIOs) as tracers. MPI has previously been shown to track therapeutic stem cells for over 87 days with a ~200 cell detection limit. In the current work, we utilized antibody-conjugated SPIOs specific to neutrophils for in situ labeling, and non-invasive and radiation-free tracking of these inflammatory cells to sites of infection and inflammation in an in vivo murine model of lipopolysaccharide-induced myositis. MPI showed sensitive detection of inflammation with a contrast-to-noise ratio of ~8-13.