Phenotypic differentiation‐linked growth inhibition in human leukemia cells by active vitamin D3 analogues
Phenotypic differentiation‐linked growth inhibition in human leukemia cells by active vitamin D3 analogues
复制标题
活性维生素 D3 类似物对人白血病细胞表型分化相关的生长抑制
DOI：
10.1002/ijc.2910330207
复制
发表时间：
1984
影响因子：
6.4
通讯作者：
T. Fujita
中科院分区：
文献类型：
--
作者：
T. Matsui;Y. Nakao;N. Kobayashi;M. Kishihara;S. Ishizuka;S. Watanabe;T. Fujita
活性维生素D3诱导在无血清培养基中培养的人早幼粒细胞白血病细胞系HL-60的表型分化。暴露于10−1 - 10−7 M 1，25-（OH）2D 3 1，24 S-（OH）2D 3或1，24 R（OH）2D 3后，通过单克隆抗体OKM 1、63 D3和Mo 2检测到的HL-60细胞的单核细胞-粒细胞相关质膜抗原（通过荧光激活细胞分选仪分析定量）呈时间和剂量依赖性增加。在暴露于1，25-（OH）2D 3后，在16 h内检测到该抗原表达的促进，并且分化诱导持续至96 h。携带单克隆抗体OKT 9识别的转铁蛋白受体的细胞数量及其在HL-60细胞表面上的密度对称地减少。这些作用与细胞生长、聚（ADP-核糖）含量和从头DNA-RNA合成的抑制平行出现。这些结果表明，1，24 S-（OH）2D 3在体外类似于1，25-（OH）2D 3促进HL-60的分化诱导，并且转铁蛋白受体的减少与增殖抑制明显相关。
Active vitamin D3 induced phenotypic differentiation of the human promyelocytic leukemia cell line, HL‐60, cultured in serum‐free medium. Upon exposure to 10−1‐10−7 M 1,25‐(OH)2D3 1,24S‐(OH)2D3 or 1,24R(OH)2D3, monocyte‐granulocyte‐associated plasma membrane antigens of HL‐60 cells detected by monoclonal antibodies, OKM1, 63D3 and Mo2, quantitated by fluorescence‐activated cell sorter analysis, were increased time‐ and dose‐dependently. After expose to 1,25‐(OH)2D3, promotion of this antigenic expression was detected within 16 h, and the induction of differentiation continued until 96 h. The number of cells bearing transferrin receptors recognized by the monoclonal antibody, OKT9, and its density on the surface of HL‐60 cells were decreased symmetrically. These effects appeared in parallel with the inhibition of cell growth, poly(ADP‐ribose) content and de novo DNA‐RNA synthesis. These findings indicate that 1,24S‐(OH)2D3 stimulates differentiating induction of HL‐60 like 1,25‐(OH)2D3 in vitro and that the decrease of transferrin receptor is apparently correlated with the inhibition of proliferation.
维生素 D 状态和刷状缘膜囊泡：1,25-二羟基维生素 D3 诱导不稳定。
DOI：
10.1002/jbmr.5650010402
发表时间：
1986
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Putkey,JA;Nemere,I;Norman,AW
通讯作者：
Norman,AW
DOI：
10.1073/pnas.77.11.6764
发表时间：
1980
影响因子：
11.1
作者：
Ugolini,V;Nunez,G;Smith,RG;Stastny,P;Capra,JD
通讯作者：
Capra,JD
通过单克隆抗体鉴定人单核细胞上的抗原。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Todd3rd,RF;Nadler,LM;Schlossman,SF
通讯作者：
Schlossman,SF