Pacemaker and plateau potentials shape output of a developing locomotor network.
Pacemaker and plateau potentials shape output of a developing locomotor network.
复制标题
DOI：
10.1016/j.cub.2012.10.025
复制
发表时间：
2012-12-18
期刊：
影响因子：
9.2
通讯作者：
McDearmid, Jonathan Robert
中科院分区：
文献类型：
--
作者：
Tong, Huaxia;McDearmid, Jonathan Robert
在发育过程中，脊髓网络经历了一个激烈的成熟期，在此期间观察到不成熟的运动行为形式。这样的行为是暂时的，随着开发的进行，会让位于更成熟的活动。控制运动行为中年龄特异性转变的过程尚未完全了解。利用体内膜片钳电生理学，我们表征了发育中的斑马鱼脊髓神经元的离子电导和放电模式。我们发现脊髓中间神经元的核，同侧尾侧（IC）细胞，产生固有的破裂活动，这取决于持续钠电流（INaP）。我们进一步表明，运动行为的发育转变伴随着IC细胞破裂的变化：在生命早期，这些细胞产生低频膜振荡，可能驱动“卷曲”，这是一种不成熟的运动输出形式。当鱼类成熟到游泳阶段时，IC细胞切换到一种持续的破裂模式，允许在运动过程中产生高频振荡。最后，我们发现集成电路细胞破裂的扰动破坏了线圈和游泳阶段的电机输出。我们的研究结果表明，具有独特爆发特征的神经元是发展运动网络的基本组成部分。在发育过程中，这些可能会形成网络输出，并促进运动行为中特定阶段的重新配置。►发育中的斑马鱼CPG的IC细胞表现出依赖于inap的破裂活动►IC细胞的破裂活动是阶段特异性的►在卷曲阶段，IC细胞产生有节奏的膜振荡►在游泳阶段，IC细胞产生高频、持续的破裂
During development, spinal networks undergo an intense period of maturation in which immature forms of motor behavior are observed. Such behaviors are transient, giving way to more mature activity as development proceeds. The processes governing age-specific transitions in motor behavior are not fully understood. Using in vivo patch clamp electrophysiology, we have characterized ionic conductances and firing patterns of developing zebrafish spinal neurons. We find that a kernel of spinal interneurons, the ipsilateral caudal (IC) cells, generate inherent bursting activity that depends upon a persistent sodium current (INaP). We further show that developmental transitions in motor behavior are accompanied by changes in IC cell bursting: during early life, these cells generate low frequency membrane oscillations that likely drive “coiling,” an immature form of motor output. As fish mature to swimming stages, IC cells switch to a sustained mode of bursting that permits generation of high-frequency oscillations during locomotion. Finally, we find that perturbation of IC cell bursting disrupts motor output at both coiling and swimming stages. Our results suggest that neurons with unique bursting characteristics are a fundamental component of developing motor networks. During development, these may shape network output and promote stage-specific reconfigurations in motor behavior. ► IC cells of the developing zebrafish CPG exhibit INaP-dependent bursting activity ► IC cell bursting activity is stage specific ► At coiling stages, IC cells generate rhythmic membrane oscillations ► At swimming stages, IC cells generate high-frequency, sustained bursting
登录
查看更多内容
影响因子：
2.5
作者：
Del Negro, CA;Koshiya, N;Smith, JC
通讯作者：
Smith, JC
影响因子：
2.5
作者：
Li, YR;Bennett, DJ
通讯作者：
Bennett, DJ
DOI：
10.1073/pnas.79.7.2415
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LLINAS, R;SUGIMORI, M;SIMON, SM
通讯作者：
SIMON, SM
影响因子：
5.3
作者：
Del Negro, CA;Morgado-Valle, C;Feldman, JL
通讯作者：
Feldman, JL
影响因子：
5.5
作者：
Kuo, J. J.;Lee, R. H.;Heckman, C. J.
通讯作者：
Heckman, C. J.