Rapid clearance of epigenetic protein reporters from wound edge cells in Drosophila larvae does not depend on the JNK or PDGFR/VEGFR signaling pathways.
Rapid clearance of epigenetic protein reporters from wound edge cells in Drosophila larvae does not depend on the JNK or PDGFR/VEGFR signaling pathways.
复制标题
果蝇幼虫中伤口边缘细胞的表观遗传蛋白报道的快速清除不取决于JNK或PDGFR/VEGFR信号通路。
DOI：
10.1002/reg2.12
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发表时间：
2014-04-01
期刊：
影响因子：
--
通讯作者：
Galko, Michael J
中科院分区：
文献类型：
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作者：
Anderson, Aimee E;Galko, Michael J
The drastic cellular changes required for epidermal cells to dedifferentiate and become motile during wound closure are accompanied by changes in gene transcription, suggesting corresponding alterations in chromatin. However, the epigenetic changes that underlie wound‐induced transcriptional programs remain poorly understood partly because a comprehensive study of epigenetic factor expression during wound healing has not been practical. To determine which chromatin modifying factors might contribute to wound healing, we screened publicly available fluorescently tagged reporter lines in Drosophila for altered expression at the wound periphery during healing. Thirteen reporters tagging seven different proteins showed strongly diminished expression at the wound edge. Three downregulated proteins, Osa, Kismet, and Spt6, are generally associated with active chromatin, while four others, Sin3A, Sap130, Mi‐2, and Mip120, are associated with repressed chromatin. In all cases reporter downregulation was independent of the Jun N‐terminal kinase and Pvr pathways, suggesting that novel signals control reporter clearance. Taken together, our results suggest that clearance of chromatin modifying factors may enable wound edge cells to rapidly and comprehensively change their transcriptional state following tissue damage.
