Pituitary adenylate cyclase-activating polypeptide enhances saliva secretion via direct binding to PACAP receptors of major salivary glands in mice.
Pituitary adenylate cyclase-activating polypeptide enhances saliva secretion via direct binding to PACAP receptors of major salivary glands in mice.
复制标题
垂体腺苷酸环化酶激活多肽通过直接结合小鼠主要唾液腺的 PACAP 受体来增强唾液分泌。
DOI：
10.1002/ar.23388
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
NAKAMURA M
中科院分区：
文献类型：
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作者：
MATOBA Y;NONAKA N;TAKAGI Y;IMAMURA E;NARUKAWA M;NAKAMACHI T;SHIODA S;BANKS WA;NAKAMURA M
口干症或口干是一种常见的综合征，通常用人工唾液治疗;然而，尚未建立其他有效的方法。唾液的分泌主要受自主神经系统的控制。腺苷酸环化酶激活多肽（PACAP）是一种多功能的神经肽，存在于多种器官中。在这项研究中，我们研究了PACAP对唾液分泌的影响，并检测了9周龄雄性C57 BL/6小鼠主要唾液腺（包括腮腺，下颌下腺和舌下腺）中PACAP 1型受体（PAC 1 R）的分布。鼻内给药PACAP 38增加唾液分泌量，阿托品预处理不抑制。免疫组化结果显示PAC 1 R在三大涎腺均有分布。在腮腺和舌下腺中，在纹状管细胞中检测到PAC 1 R，而在下颌下腺中，在颗粒管中的高柱状上皮细胞中检测到强烈的PAC 1 R免疫反应（即，柱细胞），以及在一些纹状管细胞。PACAP可显著增加唾液中表皮生长因子的浓度。这些结果表明，PACAP通过控制导管中的吸收活性直接调节唾液分泌，并且柱细胞调节颗粒导管中的颗粒上皮细胞的功能，例如将生长因子分泌到唾液中。总的来说，这些结果表明PACAP作为一种新的有效治疗口干症的可能性。Anat Rec，299：1293-1299，2016。© 2016 Wiley Periodicals，Inc.
Xerostomia, or dry mouth, is a common syndrome that is generally treated with artificial saliva; however, no other effective methods have yet been established. Saliva secretion is mainly under the control of the autonomic nervous system. Pituitary adenylate cyclase‐activating polypeptide (PACAP) is recognized as a multifunctional neuropeptide in various organs. In this study, we examined the effect of PACAP on saliva secretion, and detected the distribution of the PACAP type 1 receptor (PAC1R) in major salivary glands, including the parotid, submandibular, and sublingual glands, in 9‐week‐old male C57BL/6 mice. Intranasal administration of PACAP 38 increased the amount of saliva secreted, which was not inhibited by atropine pretreatment. Immunohistochemical analysis showed that PAC1R was distributed in the three major salivary glands. In the parotid and sublingual glands, PAC1R was detected in striated duct cells, whereas in the submandibular gland, a strong PAC1R immunoreaction was detected in tall columnar epithelial cells in the granular ducts (i.e., pillar cells), as well as in some striated duct cells. PACAP significantly increased the concentration of epidermal growth factor in saliva. These results suggest that PACAP directly regulates saliva secretion by controlling the absorption activity in the ducts, and that pillar cells regulate the function of granular epithelial cells in the granular duct, such as the secretion of growth factors into the saliva. Collectively, these results suggest the possibility of PACAP as a new effective treatment of xerostomia. Anat Rec, 299:1293–1299, 2016. © 2016 Wiley Periodicals, Inc.