The use of dentin matrix scaffold and dental follicle cells for dentin regeneration
The use of dentin matrix scaffold and dental follicle cells for dentin regeneration
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牙本质基质支架和牙囊细胞在牙本质再生中的应用
DOI：
10.1016/j.biomaterials.2009.08.034
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发表时间：
2009-12-01
期刊：
影响因子：
14
通讯作者：
Jin, Yan
中科院分区：
文献类型：
--
作者：
Guo, Weihua;He, Yong;Jin, Yan
支架和诱导微环境是牙本质再生的两个最重要的因素。这些问题已经用羟基磷灰石、磷酸三钙、聚乙醇酸、煅烧牛骨和胶原蛋白等来解决。然而，到目前为止，没有支架和诱导微环境的组合已被证明有助于完整的和预制形状的牙本质组织，包括牙本质，前牙本质和成牙本质细胞的再生。为了测试经处理的牙本质基质（TDM）对完整和预制形状的牙本质再生的支持和诱导作用，将牙囊细胞（DFC）接种到TDM上，并进一步体外培养1和2周以及体内培养2和4周。结果表明，在体外培养条件下，经TDM诱导的牙本质细胞除表达牙本质涎蛋白（DSP）和牙本质基质蛋白1（DMP1）外，还表达成牙本质细胞表达的骨钙素、骨涎蛋白、1型胶原、骨桥蛋白、骨连接素和碱性磷酸酶，并能成功再生完整的预制牙本质。最重要的是，发现体内TDM支持并诱导完整和预制形状的牙本质再生，并且再生的牙本质表达DSP和DMP1，其是识别牙本质标记物。总而言之，这些结果表明，对于牙本质再生，TDM是合适的支架，诱导微环境和DFC是合适的细胞类型。TDM和DFC的组合可能构成未来临床牙本质再生的有前途的方法。(C)2009爱思唯尔有限公司保留所有权利。
Scaffold and inductive microenvironment are the two most important factors for dentin regeneration. They have been addressed with hydroxyapatite, tricalcium phosphate, polyglycolic acid, calcined bovine bone, and collagen, among other things. However, as of yet, no scaffold and inductive microenvironment combination has been shown to contribute to the regeneration of complete and prefabricated-shaped dentin tissues that include dentin, predentin and odontoblasts. To test the supporting and inductive effects of treated dentin matrix (TDM) on complete and prefabricated-shaped dentin regeneration, dental follicle cells (DFCs) were seeded onto TDM and further incubated for 1 and 2 weeks in vitro and for 2 and 4 weeks in vivo. The results show that in vitro, in addition to dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1) (regarded as identifying markers of odontoblasts), DFCs induced by TDM expressed osteocalcin, bone sialoprotein, type 1 collagen, osteopontin, osteonectin and alkaline phosphatase (all expressed by odontoblasts), and that complete and prefabricated-shaped dentin was successfully regenerated. Most importantly, it was found that in vivo TDM supports and induces regeneration of complete and prefabricated-shaped dentin, and regenerated dentin expresses DSP and DMP1, which are identifying dentin markers. Taken together, these results suggest that, for dentin regeneration, TDM is a suitable scaffold and inductive microenvironment and DFCs are a suitable cell type. The combination of TDM and DFCs may constitute a promising approach for future clinical dentin regeneration. (C) 2009 Elsevier Ltd. All rights reserved.