A Novel Cylinder-Type Poly(L-Lactic Acid)-Collagen Hybrid Sponge for Cartilage Tissue Engineering
A Novel Cylinder-Type Poly(L-Lactic Acid)-Collagen Hybrid Sponge for Cartilage Tissue Engineering
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DOI：
10.1089/ten.tec.2008.0703
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
He, Xiaoming;Lu, Hongxu;Chen, Guoping
同时具有高孔隙率和高机械强度的多孔支架的开发对于组织工程和再生医学具有相当大的挑战性。将胶原海绵包埋在杯状聚乳酸（PLLA）海绵中，制备了一种新型的PLLA/胶原杂化海绵。胶原海绵在PLLA海绵杯的中心形成，胶原微海绵在PLLA海绵杯的孔中形成。PLLA-胶原混合海绵显示出比PLLA海绵杯和胶原海绵更高的机械强度。PLLA-胶原混合海绵的孔隙率大于PLLA海绵杯的孔隙率。杯状PLLA海绵骨架为混合海绵提供了高机械强度，并在细胞接种期间防止细胞泄漏，而中央胶原海绵有助于高孔隙率，并促进细胞在混合海绵中的粘附和分布。软骨细胞在混合海绵中培养时，软骨组织成功再生。这种杂交方法为组织工程多孔支架的制备提供了一种新的技术。
The development of porous scaffolds having both high porosity and strong mechanical strength for tissue engineering and regenerative medicine has been quite challenging. A novel hybrid poly(L-lactic acid) (PLLA) collagen hybrid sponge was developed by enclosing collagen sponge in a cup-shaped PLLA sponge to meet the necessary requirements. Collagen sponge was formed in the center of the PLLA sponge cup, and collagen microsponges were formed in the pores of the PLLA sponge cup. The PLLA-collagen hybrid sponge showed higher mechanical strength than did those of the PLLA sponge cup and collagen sponge. The porosity of the PLLA-collagen hybrid sponge was greater than that of the PLLA sponge cup. The cup-shaped PLLA sponge skeleton provided the hybrid sponge with high mechanical strength and protected against cell leakage during cell seeding, while the central collagen sponge contributed to high porosity, and facilitated cell adhesion and distribution in the hybrid sponge. Cartilaginous tissue was successfully regenerated when chondrocytes were cultured in the hybrid sponge. This method of hybridization will provide a new technique for the preparation of functional porous scaffolds for tissue engineering.