Elimination of the Schlieren effect in the determination of reactive phosphorus in estuarine waters by flow injection analysis
Elimination of the Schlieren effect in the determination of reactive phosphorus in estuarine waters by flow injection analysis
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DOI：
10.1016/s0003-2670(97)00371-1
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发表时间：
1997-09
影响因子：
6.2
通讯作者：
I. McKelvie;D. M. Peat;G. Matthews;P. Worsfold
中科院分区：
文献类型：
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作者：
I. McKelvie;D. M. Peat;G. Matthews;P. Worsfold
传统的用于测定河口沃茨中活性磷的流动注射歧管受纹影或折射率（RI）效应的限制，这会导致定量中的重大误差。一个简单的流动注射（FI）歧管，它消除了反应性磷测量中的RI误差的报告。它涉及将酸性磷酸盐试剂注入到具有相似折射率的氯化钠溶液的载流中，然后依次与样品（其盐度可能因样品而异）和还原剂合并。尽管出现了相当大的空白信号，但已成功地测量了盐度范围为0至34%的样品中的活性磷。使用在去离子水中制备的校准标准品，检测限为6 μg l−1P。
Conventional flow-injection manifolds with sample injection for the determination of reactive phosphorus in estuarine waters are limited by the Schlieren or refractive index (RI) effect which can cause major errors in quantification. A simple flow-injection (FI) manifold which obviates this RI error in reactive phosphorus measurement is reported. It involves the injection of acidic molybdophosphate reagent into a carrier stream of sodium chloride solution of similar refractive index, which is then sequentially merged with a sample (the salinity of which may vary widely from sample to sample) and a reductant. Despite the occurrence of sizeable blank signals, reactive phosphorus has been successfully measured in samples with salinities ranging from 0 to 34%. using calibration standards prepared in deionised water, with a detection limit of 6 μg l−1P.