Structure and distribution of Alu family sequences or their analogs within heterogeneous nuclear RNA of HeLa, KB, and L cells.
Structure and distribution of Alu family sequences or their analogs within heterogeneous nuclear RNA of HeLa, KB, and L cells.
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HeLa、KB 和 L 细胞异质核 RNA 中 Alu 家族序列或其类似物的结构和分布。
DOI：
10.1128/mcb.4.2.310-316.1984
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发表时间：
1984
影响因子：
5.3
通讯作者：
Dickson,E
中科院分区：
文献类型：
--
作者：
Robertson,HD;Dickson,E
We studied the distribution of repetitive sequence elements capable of forming double-stranded regions in nuclear RNA of HeLa, KB, and L cells. In human RNA populations, we called these regions duplexAlufamily RNA (dAfRNA) because they represent transcripts of the highly reiterated family of DNA regions known as “Alufamily DNA” (Rubin et al., Nature (London)284:372–374, 1980). Although the dAfRNA populations of both human cell lines (HeLa and KB) have low sequence complexity, they represent 5% of the total heterogeneous nuclear RNA and have identical fingerprints; mouse L-cell dAf-like RNA (which has a similar complexity) represents only 2% of the total heterogeneous nuclear RNA and has an entirely different fingerprint. We utilizedEscherichia coliRNase III as a highly specific reagent for the recognition of RNA:RNA duplex structure. This enzyme cleaves within the six characteristic RNase T1-resistant oligonucleotides of HeLa- and KB-cell dAfRNA (Robertson et al., J. Mol. Biol.115:571–589, 1977). In addition, the size of heterogeneous nuclear RNA from all three cell types is reduced from greater than 32S to about 15S after RNase III treatment. We conclude that this size shift is a result of cleavage within dAfRNA regions and that such regions are present in most or all of the large RNA transcripts of these cells.
人免疫球蛋白 Cγ4 基因的核苷酸序列
DOI：
--
发表时间：
1981
期刊：
影响因子：
--
作者：
J. Ellison;J. Buxbaum;L. Hood
通讯作者：
L. Hood