INDUCTION OF ENDOTHELIAL-CELL TISSUE FACTOR ACTIVITY BY SERA FROM PATIENTS WITH ANTIPHOSPHOLIPID SYNDROME - A POSSIBLE MECHANISM OF THROMBOSIS
INDUCTION OF ENDOTHELIAL-CELL TISSUE FACTOR ACTIVITY BY SERA FROM PATIENTS WITH ANTIPHOSPHOLIPID SYNDROME - A POSSIBLE MECHANISM OF THROMBOSIS
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DOI：
10.1016/s0002-9378(12)90915-1
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发表时间：
1993-01-01
影响因子：
9.8
通讯作者：
RODGERS, GM
中科院分区：
文献类型：
--
作者：
BRANCH, DW;RODGERS, GM
目的：我们的研究的假设是，抗磷脂抗体易患血栓形成诱导内皮细胞组织因子expression.STUDY设计：单层培养的人脐静脉内皮细胞在培养基中孵育8小时，其中含有20%的血清从患者抗磷脂抗体（n = 11）或正常受试者（n = 8）。用来自抗磷脂抗体患者（n = 3）或正常受试者（n = 3）的免疫球蛋白G组分进行类似孵育。内皮细胞组织因子的表达与组织因子特异性显色底物测定。结果：抗磷脂血清诱导的内皮细胞组织因子表达显著高于对照组（p < 0.02）。免疫球蛋白实验表明，负责诱导组织因子表达的因子存在于血清的免疫球蛋白G牵引中（p < 0.01）。结论：内皮细胞组织因子表达由抗磷脂血清诱导，其活性至少部分存在于免疫球蛋白组分中。抗磷脂血清诱导的组织因子可能在抗磷脂综合征患者中观察到的血栓形成倾向中发挥作用。
OBJECTIVE: The hypothesis of our study is that antiphospholipid antibodies predispose to thrombosis by inducing endothelial cell tissue factor expression.STUDY DESIGN: Monolayers of cultured human umbilical vein endothelial cells were incubated for 8 hours in a medium containing 20% serum obtained either from patients with antiphospholipid antibodies (n = 11) or normal subjects (n = 8). Similar incubations were performed with immunoglobulin G fractions from either patients with antiphospholipid antibodies (n = 3) or normal subjects (n = 3). Endothelial cell tissue factor expression was measured with a tissue factor-specific chromogenic substrate assay. The results were analyzed with a two-tailed t test.RESULTS: The mean endothelial cell tissue factor expression induced by antiphospholipid sera was significantly greater than the controls (p < 0.02). Immunoglobulin experiments indicated that the factor(s) responsible for the induction of tissue tactor expression resides in the immunoglobulin G traction of the sera (p < 0.01).CONCLUSIONS: Endothelial cell tissue factor expression is induced by antiphospholipid sera, with activity residing at least in part in the immunoglobulin fraction. The induction of tissue factor by antiphospholipid sera may play a role in the thrombotic tendency observed in patients with antiphospholipid syndrome.