A two-tailed Comet assay for assessing DNA damage in spermatozoa
A two-tailed Comet assay for assessing DNA damage in spermatozoa
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DOI：
10.1016/s1472-6483(10)60003-x
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发表时间：
2009-05-01
影响因子：
4
通讯作者：
Gosalvez, Jaime
中科院分区：
文献类型：
--
作者：
Enciso, Maria;Sarasa, Jonas;Gosalvez, Jaime
DNA 碎片被认为是精液质量的重要参数，并且作为男性生育力的预测因子具有重要价值。染色质质量差与一些生育问题密切相关，并且高度预示着一些生育问题。评估精子中 DNA 碎片的方法有很多，但它们都无法区分同一精子细胞中的单链 DNA 断裂 (SSB) 和双链 DNA 断裂 (DSB)。双尾彗星测定 (2T-Comet) 方案克服了这一限制。对原始彗星测定法进行了改进，用于同时评估人类精子中的 DNA SSB 和 DSB。 2T-Comet 测定是一种快速、灵敏且可靠的方法，用于定量和表征精子中的 DNA 损伤。它是一种评估精子 DNA 完整性的创新方法，对人类生育能力和男科病理学具有重要意义。
DNA fragmentation is considered an important parameter of semen quality, and of significant value as a predictor of male fertility. Poor quality chromatin is closely associated with, and highly indicative of, some fertility problems. Many methodologies to assess DNA fragmentation in spermatozoa are available, but they are all unable to differentiate between single-stranded DNA breaks (SSB) and double-stranded DNA breaks (DSB) in the same sperm cell. The two-tailed Comet assay (2T-Comet) protocol overcomes this limitation. A modification of the original Comet assay was developed for the simultaneous evaluation of DNA SSB and DSB in human spermatozoa. The 2T-Comet assay is a fast, sensitive, and reliable procedure for the quantification and characterization of DNA damage in spermatozoa. It is an innovative method for assessing sperm DNA integrity, which has important implications for human fertility and andrological pathology.