Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1
Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1
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DOI：
10.1016/j.canlet.2003.09.031
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发表时间：
2004-02-10
期刊：
影响因子：
9.7
通讯作者：
Beppu, Y
中科院分区：
文献类型：
--
作者：
Kawai, A;Naito, N;Beppu, Y
我们在此描述了一种新的滑膜肉瘤细胞系SYO-1的建立，该细胞系来源于一名19岁女性腹股沟发生的双相滑膜肉瘤。体外培养70代以上（24个月以上）。SYO-1细胞单层培养呈纺锤形，无明显多形性。免疫组化结果：vimentin、IV型胶原、S-100、mdm2、bcl-2、c-Met、c-Kit阳性。裸鼠植入后形成的肿瘤在组织学上表现为双相特征，由梭形细胞束区和多边形到卵形细胞致密增生区组成，偶尔形成上皮斑块，表达细胞角蛋白和EMA。SYO-1细胞染色体分析显示具有t(X;18)（p11.2;q11.2）易位特征，RT-PCR显示SYT-SSX2嵌合转录。SYO-1细胞是第一个从双相滑膜肉瘤中提取的具有肿瘤遗传和表型特征的细胞系，将有助于滑膜肉瘤的各种研究，特别是其上皮分化。(C) 2003年由爱思唯尔爱尔兰有限公司出版
We describe here the establishment of a new synovial sarcoma cell line, SYO-1, derived from a biphasic synovial sarcoma that developed in the groin of a 19-year-old female. The cell line was maintained for more than 70 passages (more than 24 months) in vitro. The SYO-1 cells in monolayer culture exhibited a spindle shape without conspicuous pleomorphism. Immunohistochemically, the cells were positive for vimentin, type IV collagen, S-100, mdm2, bcl-2, c-Met and c-Kit. Tumors developed by their implantation in nude mice histologically showed biphasic features that were composed of areas of fascicles of spindle cells and areas of compact proliferation of polygonal to ovoid cells, which occasionally formed epithelial plaque and expressed cytokeratin and EMA. SYO-1 cells harbored the characteristic t(X;18)(p11.2;q11.2) translocation by chromosome analysis and SYT-SSX2 chimeric transcript by RT-PCR. The SYO-1 cells, the first characterized cell line derived from biphasic synovial sarcoma retaining the characteristic genetic and phenotypic features of the tumor, will be useful for various investigations on synovial sarcoma, especially for its epithelial differentiation. (C) 2003 Published by Elsevier Ireland Ltd.