Membranous CD24 expression as detected by the monoclonal antibody SWA11 is a prognostic marker in non-small cell lung cancer patients.
Membranous CD24 expression as detected by the monoclonal antibody SWA11 is a prognostic marker in non-small cell lung cancer patients.
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DOI：
10.1186/s12907-015-0019-z
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发表时间：
2015
影响因子：
--
通讯作者：
Kristiansen G
中科院分区：
文献类型：
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作者：
Majores M;Schindler A;Fuchs A;Stein J;Heukamp L;Altevogt P;Kristiansen G
肺癌是世界范围内最常见的恶性肿瘤之一，死亡率很高。为了使个体化治疗方案，更好地了解分子肿瘤生物学仍有待阐明。细胞表面蛋白CD 24的表达已经被认为与非小细胞肺癌（NSCLC）患者的生存期缩短相关，然而，由于最近承认常用抗体SN 3b的表位特异性有限，CD 24免疫染色在石蜡包埋组织标本中的预后价值和适用性受到质疑。用特异性识别CD 24蛋白核心的新型CD 24抗体（克隆SWA 11）对137例原发性NSCLC病例进行免疫染色，并将所得表达数据与基于单克隆抗体SN 3b的表达谱进行比较。此外，表达数据与临床病理参数相关。分别采用Kaplan Meier估计和考克斯回归进行单变量和多变量生存分析。CD 24阳性率分别为34%和34%。21%（SN 3b）的NSCLC具有膜和/或细胞质染色模式。Kaplan-Meier分析显示，CD 24的膜表达而非胞浆表达（克隆SWA 11）与淋巴结扩散和总生存时间较短相关（均p < 0.05）。通过SN 3b抗体建立的CD 24表达与总生存期没有显著的临床病理学相关性，无论是细胞质还是细胞膜CD 24染色。用抗体克隆SWA 11检测到的膜CD 24免疫反应性可作为淋巴结扩散和总生存率较差的预后因素。此外，如果CD 24被认为是一个潜在的预后生物标志物，这些结果证实了膜和细胞质定位之间的仔细区分的重要性。
Lung cancer is one of the most common malignant neoplasms worldwide and has a high mortality rate. To enable individualized therapy regimens, a better understanding of the molecular tumor biology has still to be elucidated. The expression of the cell surface protein CD24 has already been claimed to be associated with shorter patient survival in non-small cell lung cancer (NSCLC), however, the prognostic value and applicability of CD24 immunostaining in paraffin embedded tissue specimens has been questioned due to the recent acknowledgement of restricted epitope specificity of the commonly used antibody SN3b. A cohort of 137 primary NSCLC cases was immunostained with a novel CD24 antibody (clone SWA11), which specifically recognizes the CD24 protein core and the resulting expression data were compared with expression profiles based on the monoclonal antibody SN3b. Furthermore, expression data were correlated to clinico-pathological parameters. Univariate and multivariate survival analyses were conducted with Kaplan Meier estimates and Cox regression, respectively. CD24 positivity was found in 34 % resp. 21 % (SN3b) of NSCLC with a membranous and/or cytoplasmic staining pattern. Kaplan-Meier analyses revealed that membranous, but not cytoplasmic CD24 expression (clone SWA11) was associated with lympho-nodular spread and shorter overall survival times (both p < 0.05). CD24 expression established by SN3b antibodies did not reveal significant clinicopathological correlations with overall survival, neither for cytoplasmic nor membranous CD24 staining. Membranous CD24 immunoreactivity, as detected with antibody clone SWA11 may serve as a prognostic factor for lymphonodular spread and poorer overall survival. Furthermore, these results corroborate the importance of a careful distinction between membranous and cytoplasmic localisation, if CD24 is to be considered as a potential prognostic biomarker.