Neural crest invasion is a spatially-ordered progression into the head with higher cell proliferation at the migratory front as revealed by the photoactivatable protein, KikGR
Neural crest invasion is a spatially-ordered progression into the head with higher cell proliferation at the migratory front as revealed by the photoactivatable protein, KikGR
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DOI：
10.1016/j.ydbio.2008.01.029
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发表时间：
2008-04-15
影响因子：
2.7
通讯作者：
McLennan, Rebecca
中科院分区：
文献类型：
--
作者：
Kulesa, Paul M.;Teddy, Jessica M.;McLennan, Rebecca
神经嵴细胞（NCC）侵袭是一个复杂的雕刻，单个细胞进入有组织的迁移流，导致器官发育沿脊椎动物轴。我们理解分子机制如何调节NCC迁移模式的关键是关于细胞行为的信息，然而，在活胚胎中选择性标记和分析迁移的NCC一直是一个挑战。在这里，我们采用了一种创新的体内策略，通过结合KikGR的光激活和H2B-mRFP1转染的NCC的共聚焦延时分析，来研究鸡r4迁移流中的NCC行为。我们发现r4 NCC出现的空间顺序转化为从远端到近端侵犯第二支弓。领先和落后的ncc显示相似的平均蜂窝速度和方向。令人惊讶的是，我们发现领先的ncc沿着迁徙路线扩散，并且在数量上超过了落后的ncc，比例接近3:1。一个简单的、基于细胞的计算模型再现了r4 NCC的迁移模式，并预测了入侵顺序可能被速度较慢、方向性较差的铅细胞或环境噪声打乱。我们的研究结果表明，在一个模型中，NCC的行为保持了对鳃弓的空间有序侵袭，在迁移锋和迁移后的NCC之间存在细胞增殖的差异。(C) 2008爱思唯尔公司版权所有。
Neural crest cell (NCC) invasion is a complex sculpting of individual cells into organized migratory streams that lead to organ development along the vertebrate axis. Key to our understanding of how molecular mechanisms modulate the NCC migratory pattern is information about cell behaviors, yet it has been challenging to selectively mark and analyze migratory NCCs in a living embryo. Here, we apply an innovative in vivo strategy to investigate chick NCC behaviors within the rhombomere 4 (r4) migratory stream by combining photoactivation of KikGR and confocal time-lapse analysis of H2B-mRFP1 transfected NCCs. We find that the spatial order of r4 NCC emergence translates into a distal-to-proximal invasion of the 2nd branchial arch. Lead and trailing NCCs display similar average cell speeds and directionalities. Surprisingly, we find that lead NCCs proliferate along the migratory route and grow to outnumber trailing NCCs by nearly 3 to 1. A simple, cell-based computational model reproduces the r4 NCC migratory pattern and predicts the invasion order can be disrupted by slower, less directional lead cells or by environmental noise. Our results suggest a model in which NCC behaviors maintain a spatially-ordered invasion of the branchial arches with differences in cell proliferation between the migratory front and trailing NCCs. (C) 2008 Elsevier Inc. All rights reserved.