Structure and expression of the woodchuck herpesvirus genome.
Structure and expression of the woodchuck herpesvirus genome.
复制标题
土拨鼠疱疹病毒基因组的结构和表达。
DOI：
10.1016/0042-6822(91)90053-e
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发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Ogston,CW
中科院分区：
文献类型：
--
作者：
Gilles,PN;Ogston,CW
该报告描述了最近鉴定的土拨鼠疱疹病毒（旱獭疱疹病毒：HVM）中立即早期转录的基因组结构和位置。限制性片段的交叉杂交表明 HVM 基因组包含 1.5 kb 重复单元的串联阵列。此外，末端标记和核酸外切酶实验证明重复序列位于基因组的末端。代表立即早期转录的探针杂交表明，只有单一主要种类的立即早期RNA源自HVM基因组中独特序列一端附近的区域。这些结果显示出与伽马疱疹病毒亚科第 2 组的显着相似性。然而，通过常规 Southern blot 杂交未检测到 HVM 和 γ-2 原型疱疹病毒 saimiri 之间的同源性。因此，我们建议 HVM 是疱疹病毒伽马疱疹病毒亚科的新成员。
This report describes the genome structure and location from which immediate-early transcription originates in the recently characterized woodchuck herpesvirus (herpesvirus marmota: HVM). Cross-hybridization of restriction fragments indicates that the HVM genome contains a tandem array of 1.5-kb repeat units. Additionally, terminal labeling and exonuclease experiments demonstrate that the repeated sequences lie at the termini of the genome. Hybridization of probes representing immediate-early transcription indicates that only a single predominant species of immediate-early RNA originates from a region near one end of unique sequences in the HVM genome. These results show remarkable similarity with group 2 of the gammaherpesvirinae. However, no homology was detected by conventional Southern blot hybridization between HVM and the γ-2 prototype, herpesvirus saimiri. Therefore, we propose HVM to be a new member of the gammaherpesvirinae subfamily of herpesviruses.