Taurine Attenuates Carcinogenicity in Ulcerative Colitis-Colorectal Cancer Mouse Model
Taurine Attenuates Carcinogenicity in Ulcerative Colitis-Colorectal Cancer Mouse Model
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DOI：
10.1155/2020/7935917
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发表时间：
2020-05-21
影响因子：
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通讯作者：
Murata, Mariko
中科院分区：
文献类型：
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作者：
Wang, Guifeng;Ma, Ning;Murata, Mariko
牛磺酸（2-氨基乙烷磺酸）是一种氨基酸，具有许多生理和治疗功能，包括抗炎症。然而，关于牛磺酸的抗癌作用的研究很少。我们之前的研究表明，牛磺酸对体外培养的人鼻咽癌细胞具有诱导凋亡的作用。本研究通过偶氮氧甲烷(AOM)/硫酸钠（DSS）诱导小鼠结肠癌模型，探讨牛磺酸是否具有抗癌作用。除对照组外，其余小鼠均在饮水中单次腹腔注射AOM和DSS，连续7 d，两次，间隔1周。第一次DSS治疗后，小鼠随意给予蒸馏水（模型组）或饮用水中添加牛磺酸（牛磺酸组）。对照组未见肿瘤。牛磺酸显著抑制AOM+ dss诱导的肿瘤形成。组织病理学检查显示，AOM/DSS治疗在所有小鼠中均诱导结肠癌（8/8,100%），牛磺酸显著抑制结肠癌进展（4/9,44.4%）。Ki-67染色显示，牛磺酸能显著抑制癌组织细胞的增殖。牛磺酸显著增加了细胞凋亡标志物裂解型caspase-9和肿瘤抑制蛋白PTEN的水平。这是首次在AOM/ dss诱导的结肠癌小鼠模型中证明牛磺酸在体内显著降低致癌性的研究。
Taurine (2-aminoethane-sulfonic acid) is a type of amino acids and has numerous physiological and therapeutic functions, including anti-inflammation. However, there are few studies on the anticancer action of taurine. Our previous studies have demonstrated that taurine exhibits an apoptosis-inducing effect on human nasopharyngeal carcinoma cells in vitro. In this study, we have investigated whether taurine has an anticancer effect, using azoxymethane (AOM)/sulfate sodium (DSS)- induced mouse model for colon carcinogenesis. All mice, except those in control group, received a single intraperitoneal injection of AOM and DSS in the drinking water for 7 days twice, with 1-week interval. After the first DSS treatment, mice were given distilled water (model group) or taurine in the drinking water (taurine group) ad libitum. No tumor was observed in the control group. Taurine significantly suppressed AOM+DSS-induced tumor formation. Histopathological examination revealed AOM/DSS treatment induced colon cancer in all mice (8/8, 100%), and taurine significantly inhibited the progression of colon cancer (4/9, 44.4%). Taurine significantly attenuated cell proliferation in cancer tissues detected by Ki-67 staining. Taurine significantly increased the levels of an apoptosis marker cleaved caspase-9 and tumor suppressor protein PTEN. This is the first study that demonstrated that taurine significantly reduced carcinogenicity in vivo using AOM/DSS-induced colon cancer mouse model.