Phosphatidylcholine bearing 6,6-dideuterated oleic acid: A useful solid-state 2H NMR probe for investigating membrane properties.
Phosphatidylcholine bearing 6,6-dideuterated oleic acid: A useful solid-state 2H NMR probe for investigating membrane properties.
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带有 6,6-二氘化油酸的磷脂酰胆碱：一种有用的固态 2H NMR 探针，用于研究膜特性。
DOI：
10.1016/j.bmcl.2014.11.072
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Michio Murata
中科院分区：
文献类型：
--
作者：
Jin Cui;Sebastien Lethu;Tomokazu Yasuda;Shigeru Matsuoka;Nobuaki Matsumori;Fuminori Sato;Michio Murata
脂质组织一直是脂筏研究的中心。二油酰磷脂酰胆碱（DOPC）是一种典型的不饱和脂质。很少有研究报道其在筏状膜中的热力学。在此，我们开发了一种高效的[C6-2H2]油酸合成方法，并新合成了[C6-2H2]DOPC。在筏状取向双层中，[C6-2H2]DOPC 在不同温度下表现出明显的相分离和特征相行为。它已成功用于比较鞘磷脂 (SM) 和二氢鞘磷脂 (DHSM) 膜之间的膜特性。
Lipid organization has been at the center of research on lipid rafts. Dioleoylphosphatidylcholine (DOPC) is a typical unsaturated lipid. Very few studies have reported its thermodynamics in raft-like membranes. Herein, we have developed a highly efficient synthetic method for [C6-2H2] oleic acid, and newly synthesized [C6-2H2] DOPC. In raft-like oriented bilayers, [C6-2H2] DOPC shows clear phase separation and characteristic phase behavior at various temperature. It has been successfully utilized for the comparison of membrane properties between sphingomyelin (SM) and dihydrosphingomyelin (DHSM) membranes.