Cooperative Toehold: A Mechanism To Activate DNA Strand Displacement and Construct Biosensors
Cooperative Toehold: A Mechanism To Activate DNA Strand Displacement and Construct Biosensors
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合作立足点：激活 DNA 链位移和构建生物传感器的机制
DOI：
10.1021/acs.analchem.8b01202
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发表时间：
2018-08-21
影响因子：
7.4
通讯作者：
Du, Jun
中科院分区：
文献类型：
--
作者：
Hu, Pan;Li, Mengmeng;Du, Jun
支点介导的DNA链位移在各种DNA电路、DNA机器和生物传感器的构建中已被证明是强大的。到目前为止，已经开发了许多新的支点激活机制来实现程序化的DNA链位移行为。然而，几乎所有这些支点通过共价连接方式或互补杂交策略都是不灵活的，这限制了DNA装置的多功能性。为了解决这个问题，我们开发了一种新的支点，命名为“合作支点”，以激活DNA链位移。基于碱基堆叠机制，合作支点由两个相互之间完全独立的DNA序列组成。合作的立足点使人们能够不断地调整DNA链位移的速率，以及更复杂的链位移反应。合作支点也被用作生物传感器的通用信号转换器，以定性地确定RNA和ATP。此外，协同支点介导的DNA链位移作为一种新型的特异性PCR监测系统，可以检测基因组DNA样品中的pUC18质粒，且检测限为aM。
Toehold-mediated DNA strand displacement has proven powerful in the construction of various DNA circuits, DNA machines, and biosensors. So far, many new toehold activation mechanisms have been developed to achieve programmed DNA strand displacement behaviors. However, almost all those toeholds are inflexible via either a covalently attached manner or a complementary hybridization strategy, which limit the versatility of DNA devices. To solve this problem, we developed a new toehold, named "cooperative toehold", to activate DNA strand displacement. On the basis of a base stacking mechanism, the cooperative toehold is comprised of two moieties with completely independent DNA sequences between each other. The cooperative toehold enabled one to continuously tune the rate of DNA strand displacement, as well as more sophisticated strand displacement reactions. The cooperative toehold has also been employed as a universal signal translator for biosensors to qualitatively determine RNA and ATP. Moreover, as a novel specific PCR monitoring system, cooperative toehold-mediated DNA strand displacement can detect the pUC18 plasmid in genomic DNA samples with an aM detection limit.