Centrosome-dependent asymmetric inheritance of the midbody ring in Drosophila germline stem cell division.
Centrosome-dependent asymmetric inheritance of the midbody ring in Drosophila germline stem cell division.
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DOI：
10.1091/mbc.e13-09-0541
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发表时间：
2014-01
影响因子：
3.3
通讯作者：
Yamashita YM
中科院分区：
文献类型：
--
作者：
Salzmann V;Chen C;Chiang CY;Tiyaboonchai A;Mayer M;Yamashita YM
在果蝇生殖系干细胞（GSCs）的不对称分裂过程中，中体环（MR）是不对称分离的。遗传母中心体的雄性GSCs排除MR，而遗传子中心体的雌性GSCs则继承MR。此外，干细胞身份与MR遗传方式相关。许多干细胞，包括果蝇生殖系干细胞（GSCs），不对称分裂，产生一个干细胞和一个分化子细胞。细胞质分裂通常是不对称的，即使在表面对称分裂的细胞中，在分离完成后，也只有一个子细胞继承了中体环。然而，胞质分裂的不对称性是否与细胞命运相关或具有功能相关性的研究很少。在GSC分裂过程中，MR以中心体年龄依赖的方式不对称分离：继承母中心体的雄性GSC排除MR，而继承子中心体的雌性GSC继承MR。我们进一步表明干细胞身份与MR遗传模式相关。综上所述，我们的数据表明MR并不固有地决定干细胞的身份，尽管它的典型遗传是在干性的控制下，并且可能为某些因素的不对称分离提供了一个平台。
The midbody ring (MR) is asymmetrically segregated during asymmetric divisions of germline stem cells (GSCs) in Drosophila. Male GSCs, which inherit the mother centrosome, exclude the MR, whereas female GSCs, which inherit the daughter centrosome, inherit the MR. Moreover, stem cell identity correlates with the mode of MR inheritance. Many stem cells, including Drosophila germline stem cells (GSCs), divide asymmetrically, producing one stem cell and one differentiating daughter. Cytokinesis is often asymmetric, in that only one daughter cell inherits the midbody ring (MR) upon completion of abscission even in apparently symmetrically dividing cells. However, whether the asymmetry in cytokinesis correlates with cell fate or has functional relevance has been poorly explored. Here we show that the MR is asymmetrically segregated during GSC divisions in a centrosome age–dependent manner: male GSCs, which inherit the mother centrosome, exclude the MR, whereas female GSCs, which we here show inherit the daughter centrosome, inherit the MR. We further show that stem cell identity correlates with the mode of MR inheritance. Together our data suggest that the MR does not inherently dictate stem cell identity, although its stereotypical inheritance is under the control of stemness and potentially provides a platform for asymmetric segregation of certain factors.