Anticancer and antiviral activities of Youngia japonica (L.) DC (Asteraceae, Compositae)
Anticancer and antiviral activities of Youngia japonica (L.) DC (Asteraceae, Compositae)
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DOI：
10.1016/j.jep.2004.05.004
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发表时间：
2004-09-01
影响因子：
5.4
通讯作者：
Ooi, VEC
中科院分区：
文献类型：
--
作者：
Ooi, LSM;Wang, H;Ooi, VEC
摘要采用细胞病变效应（CPE）还原法测定了杨树花（又称东方山楂）水提物和乙醇提物对人早幼粒细胞白血病（HL-60）、人骨髓性白血病（慢性K-562）和小鼠肉瘤180 （S-180）三种细胞系的体外抗肿瘤活性，以及对呼吸道合胞病毒（RSV）、甲型流感病毒（Flu A）和单纯疱疹病毒1型（HSV-1）的抗病毒活性。热水浸提液对各肿瘤细胞系的细胞增殖和生长均有不同程度的抑制作用。K-562细胞对提取物最敏感，S-180细胞对提取物最不敏感。当浓度达到450马克杯/毫升时，对正常哺乳动物Vero细胞没有明显的细胞毒作用。粳稻全株乙醇提取物对HEp-2细胞培养的RSV具有抗病毒活性，但对甲型流感和HSV-1无抗病毒活性。95%乙醇提取物部分纯化的fr10和fr11具有显著的抗rsv活性，IC50在3.0 ~ 6.0 μ g/mL范围内为50%。在没有（病毒控制）和存在Fr.10和Fr.11的最大非细胞毒性浓度（MNCC）的情况下，病毒滴度降低的比例估计都为1 × 10(4) （RF，病毒滴度降低因子），表明它们的抗rsv活性足够高，值得进一步分析。初步分析表明，经化学测试，抗病毒成分可能含有包括单宁在内的酚类化合物。2004爱思唯尔爱尔兰有限公司版权所有。
Aqueous and ethanol extracts of Youngia japonica (also known as Oriental hawksbeard) were tested in vitro for anti-tumor activity against three cell lines, human promyelocytic leukaemia (HL-60), human myelogenous leukaemia (chronic K-562) and mouse Sarcoma 180 (S-180), and for antiviral activity against respiratory syncytial virus (RSV), influenza A virus (Flu A) and herpes simplex virus type 1 (HSV-1) by cytopathic effect (CPE) reduction assay. Hot water extract of Youngia japonica inhibited cell proliferation and growth of all cancer cell lines to various extent. K-562 cells were the most sensitive to the extract whereas S-180 cells were the least. It did not show any significant cytotoxic effects on normal mammalian Vero cells up to the concentration of 450 mug/mL. The ethanol extract of whole plant of Youngia japonica exhibited antiviral activity against RSV cultured in HEp-2 cells, but did not have any activity against Flu A and HSV-1. Two partially purified fractions (Fr.10 and Fr.11) from the 95% ethanol extract exhibited significant anti-RSV with 50% inhibitory concentration (IC50) in the range of 3.0-6.0 mug/mL. The ratio of the viral titer reduction in the absence (viral control) and presence of the maximal non-cytotoxic concentration (MNCC) of the Fr.10 and Fr.11 was both estimated to be 1 x 10(4) (RF, viral titer reduction factors), indicating that their anti-RSV activity was high enough to justify for further analysis. Our preliminary analysis showed that the antiviral ingredients were likely to contain phenolic compounds including tannins by chemical tests. (C) 2004 Elsevier Ireland Ltd. All rights reserved.