A Lactobacillus plantarum Esterase Active on a Broad Range of Phenolic Esters
A Lactobacillus plantarum Esterase Active on a Broad Range of Phenolic Esters
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DOI：
10.1128/aem.00323-15
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发表时间：
2015-05-01
影响因子：
4.4
通讯作者：
Munoz, Rosario
中科院分区：
文献类型：
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作者：
Esteban-Torres, Maria;Maria Landete, Jose;Munoz, Rosario
植物乳杆菌是最常见于植物来源的食品发酵中的乳酸菌种，其上富含酚类化合物。L. plantarum菌株在其适应不同环境和生长基质的能力方面显示出极大的灵活性。28 L.对植物菌株进行分析，只有7种菌株的培养物能够水解羟基肉桂酸酯，例如阿魏酸甲酯或咖啡酸甲酯。经PCR检测，只有这7株菌株具有est_1092基因。将est_1092基因导入L. plantarum WCFS1或L. lactis MG 1363，它们的培养物获得了降解羟基肉桂酸酯的能力。这些结果支持了Est_1092是L. plantarum菌株分析。重组产生Est_1092蛋白并进行生化表征。令人惊讶的是，Est_1092不仅能够水解羟基肉桂酸酯，因为所有测定的酚酯都被水解。定量PCR实验表明，est_1092的表达诱导阿魏酸甲酯，羟基肉桂酸酯的存在下，但抑制没食子酸甲酯，羟基苯甲酸酯。由于Est_1092是一种对广泛的酚酯具有活性的酶，同时具有阿魏酸酯酶和单宁酶活性，因此它在一些L. plantarum菌株为它们提供了在植物环境中存活和生长的额外优势。
Lactobacillus plantarum is the lactic acid bacterial species most frequently found in the fermentation of food products of plant origin on which phenolic compounds are abundant. L. plantarum strains showed great flexibility in their ability to adapt to different environments and growth substrates. Of 28 L. plantarum strains analyzed, only cultures from 7 strains were able to hydrolyze hydroxycinnamic esters, such as methyl ferulate or methyl caffeate. As revealed by PCR, only these seven strains possessed the est_1092 gene. When the est_1092 gene was introduced into L. plantarum WCFS1 or L. lactis MG1363, their cultures acquired the ability to degrade hydroxycinnamic esters. These results support the suggestion that Est_1092 is the enzyme responsible for the degradation of hydroxycinnamic esters on the L. plantarum strains analyzed. The Est_1092 protein was recombinantly produced and biochemically characterized. Surprisingly, Est_1092 was able to hydrolyze not only hydroxycinnamic esters, since all the phenolic esters assayed were hydrolyzed. Quantitative PCR experiments revealed that the expression of est_1092 was induced in the presence of methyl ferulate, an hydroxycinnamic ester, but was inhibited on methyl gallate, an hydroxybenzoic ester. As Est_1092 is an enzyme active on a broad range of phenolic esters, simultaneously possessing feruloyl esterase and tannase activities, its presence on some L. plantarum strains provides them with additional advantages to survive and grow on plant environments.