Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta)
Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta)
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南极科利菌（Klebsormidiales，绿藻门）细胞壁的超微结构、化学成分和生物合成
DOI：
10.1080/09670260010001735931
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
C. Andreoli
中科院分区：
文献类型：
--
作者：
G. Piro;M. Lenucci;G. Dalessandro;N. La Rocca;N. Rascio;I. Moro;C. Andreoli
本文对1989-1990年夏季采自意大利罗斯海Terra Nova站的一种绿色微生物Koliella ananastritica（Klebsormidiales，Chlorophyta）的化学组成、生物合成和细胞壁超微结构进行了研究。将纯化的微生物细胞壁依次用化学品处理，所述化学品在高等植物中溶解基质多糖（果胶和半纤维素）并留下被认为是α-纤维素的不溶性残余物。CDTA加Na 2CO 3-和KOH-溶解的多糖由葡萄糖基残基（大于90摩尔%），以及一些次要糖（甘露糖和鼠李糖）。 基质多糖的连接分析和酶水解表明存在β-1，4-连接的葡聚糖和少量其他未表征的多糖。不溶性残留物由少量与甘露聚糖或葡甘露聚糖链缔合的结晶α-纤维素组成。通过在D-[U-14 C]葡萄糖或肌-[U-14 C]肌醇存在下培养微藻来研究细胞壁多糖的生物合成。放射性糖基残基纳入从纯化的细胞壁的培养microphones的多糖溶解对应的细胞壁的化学分析中检测到的。肌醇氧化途径被证明是功能性的，在显微镜下。细胞壁超微结构观察表明，在一个扩展的矩阵纤维素微纤丝松散的网络。
The chemical composition, biosynthesis and ultrastructural organization of the cell wall of cultures of Koliella antarctica (Klebsormidiales, Chlorophyta), a green microalga obtained from samples of sea water taken near the Italian Station of Terra Nova (Ross Sea) during the austral summer 1989–1990, have been studied. Purified cell walls of the microalga were sequentially treated with chemicals which, in higher plants, solubilize matrix polysaccharides (pectins and hemicelluloses) and leave an insoluble residue considered to be α-cellulose. CDTA plus Na2CO3- and KOH-solubilized polysaccharides were made up of glucosyl residues (more than 90 mol%), as well as some minor sugars (mannose and rhamnose). Linkage analysis and enzymic hydrolysis of the matrix polysaccharides indicated the presence of β-1,4-linked glucans with minor amounts of other uncharacterized polysaccharides. The insoluble residue was composed of a small amount of crystalline α-cellulose associated with mannan or glucomannan chains. The biosynthesis of cell wall polysaccharides was studied by incubating the microalgae in the presence of D-[U-14C]glucose or myo-[U-14C]inositol. The radioactive glycosyl residues incorporated into the polysaccharides solubilized from purified cell walls of the cultured microalga corresponded to those detected in the chemical analysis of the cell wall. The myo-inositol oxidation pathway was demonstrated to be functional in the microalga. Cell wall ultrastructural observations showed a loose network of cellulose microfibrils in an extended matrix.