Bronchointerstitial Pneumonia in Guinea Pigs Following Inoculation with H5N1 High Pathogenicity Avian Influenza Virus
Bronchointerstitial Pneumonia in Guinea Pigs Following Inoculation with H5N1 High Pathogenicity Avian Influenza Virus
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DOI：
10.1354/vp.46-1-138
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发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Swayne, D. E.
中科院分区：
文献类型：
--
作者：
Kwon, Y. K.;Lipatov, A. S.;Swayne, D. E.
H5 N1高致病性禽流感（HPAI）病毒已在亚洲、非洲和中东引起广泛的家禽疾病，并引起零星的人类感染。豚鼠模型已被用于研究人类H3 N2和H1N1流感病毒，但缺乏对H5 N1高致病性禽流感病毒感染的认识。用A/ Vletnam/1203/04（VN/04）或A/番鸭/越南/209/05（MDk/VN/05）病毒鼻内或胃内接种豚鼠。在鼻内接种VN/04病毒的豚鼠中，接种后2天和3天（DPI）观察到轻度无精打采。在5 DPI时，豚鼠出现支气管间质性肺炎，并在细支气管上皮和肺泡巨噬细胞中鉴定出病毒。从肺中分离出病毒，但从其他器官中缺乏。在鼻内MDk/VN/06组中观察到最小肺部病变，未检出病毒，但观察到感染的血清学证据。胃内暴露未能产生感染或病变的病毒。感染H5 N1病毒的豚鼠出现的局部呼吸道疾病与感染H3 N2和H1N1流感的人类非常相似，但没有报告的H5 N1人类病例严重。
The H5N1 high-pathogenicity avian influenza (HPAI) viruses have caused widespread disease Of Poultry in Asia, Africa and the Middle East, and sporadic human infections. The guinea pig model has been used to Study human H3N2 and H1N1 influenza viruses, but knowledge is lacking oil H5N1 HPAI Virus infections. Guinea pigs Were inoculated intranasally or intragastrically with A/ Vletnam/1203/04 (VN/04) or A/Muscovy duck/Vietnam/209/05 (MDk/VN/05) Viruses. Mild listlessness was seen Lit 2 and 3 days postinoculation (DPI) in guinea Pigs Inoculated intranasally with VN/04 virus. At 5 DPI, the guinea pigs had bronchointerstitial pneumonia and virus was identified ill bronchiolar epithelium and alveolar macrophages. Virus was isolated from the lungs but was lacking from other organs. Minimal lung lesions were seen in intranasal MDk/VN/06 group and virus was not detected, but serologic evidence of infection was observed. Intragastric exposure failed to produce infection or lesions with either virus. The localized respiratory disease in guinea pigs with H5N1 viruses was very similar to that of H3N2 and H1N1 influenza ill humans and was less severe than reported for H5N1 human cases.